Degradation of human Lipin-1 by BTRC E3 ubiquitin ligase

Kenji Ishimoto, Ayaka Hayase, Fumiko Kumagai, Megumi Kawai, Hiroko Okuno, Nobumasa Hino, Yoshiaki Okada, Takeshi Kawamura, Toshiya Tanaka, Takao Hamakubo, Juro Sakai, Tatsuhiko Kodama, Keisuke Tachibana, Takefumi Doi

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Lipin-1 has dual functions in the regulation of lipid and energy metabolism according to its subcellular localization, which is tightly controlled. However, it is unclear how Lipin-1 degradation is regulated. Here, we demonstrate that Lipin-1 is degraded through its DSGXXS motif. We show that Lipin-1 interacts with either of two E3 ubiquitin ligases, BTRC or FBXW11, and that this interaction is DSGXXS-dependent and mediates the attachment of polyubiquitin chains. Further, we demonstrate that degradation of Lipin-1 is regulated by BTRC in the cytoplasm and on membranes. These novel insights into the regulation of human Lipin-1 stability will be useful in planning further studies to elucidate its metabolic processes.

Original languageEnglish
Pages (from-to)159-164
Number of pages6
JournalBiochemical and biophysical research communications
Volume488
Issue number1
DOIs
Publication statusPublished - 2017 Jun 17
Externally publishedYes

Keywords

  • BTRC
  • Degradation
  • Lipid metabolism
  • Lipin-1
  • Polyubiquitination
  • Protein localization

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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