Defects of the rat premaxilla as a model of alveolar clefts for testing bone-inductive agents

Teruko Takano-Yamamoto, Masayoshi Kawakami, Mamoru Sakuda

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12 Citations (Scopus)


Critical-sized defects were made in the premaxillary bone of Wistar male rats using a surgical trephine and a low-speed dental engine as a model of the maxillary alveolar cleft for testing bone-inductive agents. The defects were treated with either 7 mg of demineralized bone matrix (DBM) or were left nongrafted. The nongrafted group healed mainly with fibrous connective tissue, with a small amount of bone formation at the periphery. There was no significant change in alkaline phosphatase activity and 45Ca incorporation. The DBM-grafted group produced new bone with osseous bridging in the defect by day 35. Alkaline phosphatase activity increased significantly from day 10, reaching a maximum on day 14, and 45Ca incorporation increased on day 14. These results indicate that this nonhealing bony wound of the premaxilla in rats may be useful as a model for studying the effect of bone-inductive agents on the healing of alveolar clefts.

Original languageEnglish
Pages (from-to)887-891
Number of pages5
JournalJournal of Oral and Maxillofacial Surgery
Issue number8
Publication statusPublished - 1993

ASJC Scopus subject areas

  • Surgery
  • Oral Surgery
  • Otorhinolaryngology


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