Defective mononuclear cell antibody-dependent cellular cytotoxicity (adcc) in patients with leukocyte adhesion deficiency emphasizing on different cd11/cd18 requirement of fcγri versus fcγrii in adcc

Toshiro Majima, Yoshiyuki Ohashi, Ryoichi Nagatomi, Atsuo Iizuka, Tasuke Konno

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

The defective antibody-dependent cellular cytotoxicity (ADCC) of mononuclear cells (MNC) from patients with leukocyte adhesion deficiency (LAD), β2integrins (CD11a-c/CD18) deficiency was shown. LAD patients completely failed to generate MNC-ADCC against sheep red blood cells (SRBC) sensitized with murine (m) IgG2b, but had diminished but significant cytolysis against mIgG2a-SRBC, suggesting that the CD11/CD18 requirement of FcγRI is different from that of FcγRII in MNC-ADCC. Blocking experiments with monoclonal antibodies (mAb) against individual subunits of CD11/CD18 revealed that anti-CD18 mAb almost completely inhibited mlgG2b-mediated ADCC by normal monocytes, but only partially inhibited mlgG2a-mediated ADCC. These data may confirm the evidence that FcγRII-mediated ADCC absolutely requires CD11/CD18 but FcγRI-mediated ADCC does not. Among subunits of CD11/CD18, CD18 appeared to be most involved in lysis of sensitized SRBC.

Original languageEnglish
Pages (from-to)385-396
Number of pages12
JournalCellular Immunology
Volume148
Issue number2
DOIs
Publication statusPublished - 1993 May

ASJC Scopus subject areas

  • Immunology

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