Decreased IL-10 production by psoriatic peripheral blood mononuclear cells stimulated with streptococcal superantigen

Setsuya Aiba, Zia Uddin, Satoshi Nakagawa, Syunji Sugawara, Hidemi Rikiishi, Katsuo Kumagai, Hachiro Tagami

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The strong association of acute guttate psoriasis and streptococcal throat infection has suggested a role for streptococcal antigens in the pathogenesis of psoriasis. We have reported that psoriatic peripheral blood mononuclear cells (PBMCs) showed significantly lower responses to cytoplasmic membrane-associated protein (CAP) isolated from group A β-hemolytic streptococci, a kind of streptococcal superantigen. The objectives were to evaluate the abnormal cytokine production by psoriatic PBMCs to streptococcal superantigen, CAP. We compared the production of four different cytokines, i.e. IL-4, IL-5, IL-10, and IFN-γ, by PBMCs between psoriatic patients and healthy controls after stimulation with CAP or two different staphylococcal superantigens, staphylococcal enterotoxin A (SEA) or E (SEE). When PBMCs were stimulated with CAP, the production of IL-10 was significantly lower by psoriatic PBMCs than by those from healthy controls, whereas those of IL-4, IL-5, or IFN-γ were not different between the two groups. Such a significant decrease in IL-10 production by psoriatic PBMCs was not observed when they were stimulated with staphylococcal superantigens. Flow cytometric analysis of intracytoplasmic IL-10 demonstrated defective IL-10 production by psoriatic PBMCs in both CD3+ T cells and CD14+ monocytes. There was a significant positive correlation between IFN-γ production by PBMCs and the proliferation of Vβ8+ T cells preferentially stimulated by CAP. These data demonstrating the defective IL-10 production by psoriatic PBMCs stimulated with streptococcal superantigen seem to explain why only psoriatic patients evolve sustained and Th-1 deviated skin lesions after streptococcal upper respiratory infection.

Original languageEnglish
Pages (from-to)337-343
Number of pages7
JournalExperimental Dermatology
Volume11
Issue number4
DOIs
Publication statusPublished - 2002 Aug

Keywords

  • Interferon-γ
  • Psoriasis
  • Streptococcus infection
  • Superantigen

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

Fingerprint Dive into the research topics of 'Decreased IL-10 production by psoriatic peripheral blood mononuclear cells stimulated with streptococcal superantigen'. Together they form a unique fingerprint.

Cite this