Decrease in fungal biodiversity along an available phosphorous gradient in arable Andosol soils in Japan

Zhihua Bao, Yuko Matsushita, Sho Morimoto, Yuko Takada Hoshino, Chika Suzuki, Kazunari Nagaoka, Makoto Takenaka, Hiroharu Murakami, Yukiko Kuroyanagi, Yasufumi Urashima, Hiroyuki Sekiguchi, Atsuhiko Kushida, Koki Toyota, Masanori Saito, Seiya Tsushima

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Andosols comprise one of the most important soil groups for agricultural activities in Japan because they cover about 46.5% of arable upland fields. In this soil group, available phosphorus (P) is accumulated by application of excessive fertilizer, but little is known about the influence of increasing P availability on microbial community diversity at large scales. We collected soil samples from 9 agro-geographical sites with Andosol soils across an available P gradient (2048.1-59.1 mg P2O5·kg-1) to examine the influence of P availability on the fungal community diversity. We used polymerase chain reaction - denaturing gradient gel electrophoresis to analyze the fungal communities based on 18S rRNA genes. Statistical analyses revealed a high negative correlation between available P and fungal diversity (H′). Fungal diversity across all sites exhibited a significant hump-shaped relationship with available P (R2 = 0.38, P < 0.001). In addition, the composition of the fungal community was strongly correlated with the available P gradient. The ribotype F6, which was positively correlated with available P, was closely related to Mortierella. The results show that both the diversity and the composition of the fungal community were influenced by available P concentrations in Andosols, at a large scale. This represents an important step toward understanding the processes responsible for the maintenance of fungal diversity in Andosolic soils.

Original languageEnglish
Pages (from-to)368-373
Number of pages6
JournalCanadian Journal of Microbiology
Volume59
Issue number6
DOIs
Publication statusPublished - 2013 Jun 1

Keywords

  • Agriculture
  • Andosols
  • Available phosphorus
  • Fertilization
  • Fungal diversity
  • PCR-DGGE

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Genetics

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