TY - JOUR
T1 - Decrease in fungal biodiversity along an available phosphorous gradient in arable Andosol soils in Japan
AU - Bao, Zhihua
AU - Matsushita, Yuko
AU - Morimoto, Sho
AU - Hoshino, Yuko Takada
AU - Suzuki, Chika
AU - Nagaoka, Kazunari
AU - Takenaka, Makoto
AU - Murakami, Hiroharu
AU - Kuroyanagi, Yukiko
AU - Urashima, Yasufumi
AU - Sekiguchi, Hiroyuki
AU - Kushida, Atsuhiko
AU - Toyota, Koki
AU - Saito, Masanori
AU - Tsushima, Seiya
PY - 2013/6/1
Y1 - 2013/6/1
N2 - Andosols comprise one of the most important soil groups for agricultural activities in Japan because they cover about 46.5% of arable upland fields. In this soil group, available phosphorus (P) is accumulated by application of excessive fertilizer, but little is known about the influence of increasing P availability on microbial community diversity at large scales. We collected soil samples from 9 agro-geographical sites with Andosol soils across an available P gradient (2048.1-59.1 mg P2O5·kg-1) to examine the influence of P availability on the fungal community diversity. We used polymerase chain reaction - denaturing gradient gel electrophoresis to analyze the fungal communities based on 18S rRNA genes. Statistical analyses revealed a high negative correlation between available P and fungal diversity (H′). Fungal diversity across all sites exhibited a significant hump-shaped relationship with available P (R2 = 0.38, P < 0.001). In addition, the composition of the fungal community was strongly correlated with the available P gradient. The ribotype F6, which was positively correlated with available P, was closely related to Mortierella. The results show that both the diversity and the composition of the fungal community were influenced by available P concentrations in Andosols, at a large scale. This represents an important step toward understanding the processes responsible for the maintenance of fungal diversity in Andosolic soils.
AB - Andosols comprise one of the most important soil groups for agricultural activities in Japan because they cover about 46.5% of arable upland fields. In this soil group, available phosphorus (P) is accumulated by application of excessive fertilizer, but little is known about the influence of increasing P availability on microbial community diversity at large scales. We collected soil samples from 9 agro-geographical sites with Andosol soils across an available P gradient (2048.1-59.1 mg P2O5·kg-1) to examine the influence of P availability on the fungal community diversity. We used polymerase chain reaction - denaturing gradient gel electrophoresis to analyze the fungal communities based on 18S rRNA genes. Statistical analyses revealed a high negative correlation between available P and fungal diversity (H′). Fungal diversity across all sites exhibited a significant hump-shaped relationship with available P (R2 = 0.38, P < 0.001). In addition, the composition of the fungal community was strongly correlated with the available P gradient. The ribotype F6, which was positively correlated with available P, was closely related to Mortierella. The results show that both the diversity and the composition of the fungal community were influenced by available P concentrations in Andosols, at a large scale. This represents an important step toward understanding the processes responsible for the maintenance of fungal diversity in Andosolic soils.
KW - Agriculture
KW - Andosols
KW - Available phosphorus
KW - Fertilization
KW - Fungal diversity
KW - PCR-DGGE
UR - http://www.scopus.com/inward/record.url?scp=84878775260&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84878775260&partnerID=8YFLogxK
U2 - 10.1139/cjm-2012-0612
DO - 10.1139/cjm-2012-0612
M3 - Article
C2 - 23750950
AN - SCOPUS:84878775260
VL - 59
SP - 368
EP - 373
JO - Canadian Journal of Microbiology
JF - Canadian Journal of Microbiology
SN - 0008-4166
IS - 6
ER -