TY - JOUR
T1 - Decrease in exhaled hydrogen as marker of congestive heart failure
AU - Shibata, Atsushi
AU - Sugano, Yasuo
AU - Shimouchi, Akito
AU - Yokokawa, Tetsuro
AU - Jinno, Naoya
AU - Kanzaki, Hideaki
AU - Ohta-Ogo, Keiko
AU - Ikeda, Yoshihiko
AU - Okada, Hideshi
AU - Aiba, Takeshi
AU - Kusano, Kengo
AU - Shirai, Mikiyasu
AU - Ishibashi-Ueda, Hatsue
AU - Yasuda, Satoshi
AU - Ogawa, Hisao
AU - Anzai, Toshihisa
N1 - Funding Information:
Funding This work was supported by a Grant-in-Aid for Scientific Research from the Japanese Society for the Promotion of Science Kakenhi Grant number 2646098 to YS; number 15K19401 to TY; Kakenhi Grant number 2511176 to NJ; Kakenhi Grant number 26253037 to AShim; the Intramural Research Fund of the National Cerebral and Cardiovascular Research Center 25-2-1 to AShim; and the Center of Innovation, Science and Technology based Radical Innovation and Entrepreneurship Program, Japan, to AShim.
Publisher Copyright:
© 2018 Author(s) (or their employer(s)).
PY - 2018
Y1 - 2018
N2 - Objective: Hydrogen excretion is thought to be related to systemic antioxidation activity. H2 selectively reduces the hydroxyl radical of free hydrogen (·OH), a highly cytotoxic form of reactive oxygen species, in cultured cells. Methods: We investigated whether exhaled H2 decreased during night sleep, reflected ·OH production and was associated with heart failure severity. We enrolled 108 patients with chronic heart failure (CHF) and 15 control participants without CHF. H2 concentration was measured by gas chromatography in exhaled breath collected before sleep and in the morning after overnight fasting. Overnight change in H2 concentration (δH2) was calculated. Mitochondrial morphology evaluated by transmission electron microscopy in endomyocardial biopsies collected from 18 patients with dilated cardiomyopathy. Results: δH2 was significantly lower in patients with CHF compared with controls (-4.3±1.0 vs 2.0±2.1 ppm, p=0.030) and was positively correlated with cardiac index (CI; r = -0.285, p=0.003). Patients with a δH20 ppm had a significantly lower CI compared with those who had a δH2 >0 ppm (2.85±0.61 vs 3.24±0.65 L/min/m2, p=0.005). δH2 was negatively correlated with both the percentage of vacuole-containing mitochondria and indices of cristae remodelling (r = -0.61, p=0.007). Conclusions: Decrease in exhaled H2 during night sleep was associated with CHF severity. δH2 warrants investigation as marker of CHF severity.
AB - Objective: Hydrogen excretion is thought to be related to systemic antioxidation activity. H2 selectively reduces the hydroxyl radical of free hydrogen (·OH), a highly cytotoxic form of reactive oxygen species, in cultured cells. Methods: We investigated whether exhaled H2 decreased during night sleep, reflected ·OH production and was associated with heart failure severity. We enrolled 108 patients with chronic heart failure (CHF) and 15 control participants without CHF. H2 concentration was measured by gas chromatography in exhaled breath collected before sleep and in the morning after overnight fasting. Overnight change in H2 concentration (δH2) was calculated. Mitochondrial morphology evaluated by transmission electron microscopy in endomyocardial biopsies collected from 18 patients with dilated cardiomyopathy. Results: δH2 was significantly lower in patients with CHF compared with controls (-4.3±1.0 vs 2.0±2.1 ppm, p=0.030) and was positively correlated with cardiac index (CI; r = -0.285, p=0.003). Patients with a δH20 ppm had a significantly lower CI compared with those who had a δH2 >0 ppm (2.85±0.61 vs 3.24±0.65 L/min/m2, p=0.005). δH2 was negatively correlated with both the percentage of vacuole-containing mitochondria and indices of cristae remodelling (r = -0.61, p=0.007). Conclusions: Decrease in exhaled H2 during night sleep was associated with CHF severity. δH2 warrants investigation as marker of CHF severity.
KW - heart failure
KW - Idiopathic dilated cardiomyopathy
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U2 - 10.1136/openhrt-2018-000814
DO - 10.1136/openhrt-2018-000814
M3 - Article
AN - SCOPUS:85056289707
VL - 5
JO - Open Heart
JF - Open Heart
SN - 2053-3624
IS - 2
M1 - e000814
ER -