Decrease in cytosine methylation at CpG island shores and increase in DNA fragmentation during zebrafish aging

Nobuyoshi Shimoda, Toshiaki Izawa, Akio Yoshizawa, Hayoto Yokoi, Yutaka Kikuchi, Naohiro Hashimoto

    Research output: Contribution to journalArticlepeer-review

    27 Citations (Scopus)

    Abstract

    Age-related changes in DNA methylation have been demonstrated in mammals, but it remains unclear as to the generality of this phenomenon in vertebrates, which is a criterion for the fundamental cause of senescence. Here we showed that the zebrafish genome gradually and clearly lost methylcytosine in somatic cells, but not in male germ cells during aging, and that age-dependent hypomethylation preferentially occurred at a particular domain called the CpG island shore,which is associated with vertebrates' genes and has been shown to be hypomethylated in humans with age. We also found that two CpG island shores hypomethylated in zebrafish oocytes were de novo methylated in fertilized eggs, which suggests that the zebrafish epigenome is reset upon fertilization, enabling new generations to restart with a heavily methylated genome. Furthermore, we observed an increase in cleavage of the zebrafish genome to an oligonucleosome length in somatic cells from the age of 12 months, which is suggestive of an elevated rate of apoptosis in the senescent stage.

    Original languageEnglish
    Pages (from-to)103-115
    Number of pages13
    JournalAge
    Volume36
    Issue number1
    DOIs
    Publication statusPublished - 2014 Feb

    Keywords

    • Aging
    • CpG island shore
    • DNA methylation
    • Zebrafish

    ASJC Scopus subject areas

    • Ageing
    • Geriatrics and Gerontology

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