TY - JOUR
T1 - Decomposition of glucose-sensitive layer-by-layer films using hemin, DNA, and glucose oxidase
AU - Yoshida, Kentaro
AU - Kashimura, Yu
AU - Kamijo, Toshio
AU - Ono, Tetsuya
AU - Dairaku, Takenori
AU - Sato, Takaya
AU - Kashiwagi, Yoshitomo
AU - Sato, Katsuhiko
N1 - Funding Information:
Funding: This work was supported Kakenhi Grants-in-Aid (Nos. JP18K06791 and JP18K19936) from the Japan Society for the Promotion of Science (JSPS) and the Ohu University Research Fund.
Publisher Copyright:
© 2020 by the authors.
PY - 2020/2/1
Y1 - 2020/2/1
N2 - Glucose-sensitive films were prepared through the layer-by-layer (LbL) deposition of hemin-modified poly(ethyleneimine) (H-PEI) solution and DNA solution (containing glucose oxidase (GOx). H-PEI/DNA + GOx multilayer films were constructed using electrostatic interactions. The (H-PEI/DNA + GOx)5 film was then partially decomposed by hydrogen peroxide (H2O2). The mechanism for the decomposition of the LbL film was considered to involve more reactive oxygen species (ROS) that were formed by the reaction of hemin and H2O2, which then caused nonspecific DNA cleavage. In addition, GOx present in the LbL films reacts with glucose to generate hydrogen peroxide. Therefore, decomposition of the (H-PEI/DNA + GOx)5 film was observed when the thin film was immersed in a glucose solution. (H-PEI/DNA + GOx)5 films exposed to a glucose solution for periods of 24, 48 72, and 96 h indicated that the decomposition of the film increased with the time to 9.97%, 16.3%, 23.1%, and 30.5%, respectively. The rate of LbL film decomposition increased with the glucose concentration. At pH and ionic strengths close to physiological conditions, it was possible to slowly decompose the LbL film at low glucose concentrations of 1-10 mM.
AB - Glucose-sensitive films were prepared through the layer-by-layer (LbL) deposition of hemin-modified poly(ethyleneimine) (H-PEI) solution and DNA solution (containing glucose oxidase (GOx). H-PEI/DNA + GOx multilayer films were constructed using electrostatic interactions. The (H-PEI/DNA + GOx)5 film was then partially decomposed by hydrogen peroxide (H2O2). The mechanism for the decomposition of the LbL film was considered to involve more reactive oxygen species (ROS) that were formed by the reaction of hemin and H2O2, which then caused nonspecific DNA cleavage. In addition, GOx present in the LbL films reacts with glucose to generate hydrogen peroxide. Therefore, decomposition of the (H-PEI/DNA + GOx)5 film was observed when the thin film was immersed in a glucose solution. (H-PEI/DNA + GOx)5 films exposed to a glucose solution for periods of 24, 48 72, and 96 h indicated that the decomposition of the film increased with the time to 9.97%, 16.3%, 23.1%, and 30.5%, respectively. The rate of LbL film decomposition increased with the glucose concentration. At pH and ionic strengths close to physiological conditions, it was possible to slowly decompose the LbL film at low glucose concentrations of 1-10 mM.
KW - Glucose sensitive
KW - Hydrogen peroxide response
KW - Layer-by-layer
KW - Multilayer thin film
KW - Stimuli-sensitive
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U2 - 10.3390/polym12020319
DO - 10.3390/polym12020319
M3 - Article
AN - SCOPUS:85081277394
SN - 2073-4360
VL - 12
JO - Polymers
JF - Polymers
IS - 2
M1 - 319
ER -