De novo expression of aromatase in gastric carcinoma: Light and electron microscopic immunohistochemical and Immunoblot study

Y. Saitoh, H. Sasano, H. Naganuma, H. Ohtani, N. Sasano, A. Ohuchi, S. Matsuno

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

We have and studies s of steroidogenic enzymes involved in estrogen biosynthesis in 30 cases of gastric carcinoma in order to investigate possible in situ production of estradiol (E 2) in carcinoma cells. Positive performed immunobistochemical immunochemical incidenc~ of immunoreactivity for E 2, testosterone (T), cholesterol side-chain cleavage enzyme (P-4S0acc) and o,nrmatase (P4SOa,on) were 27BO (S6.7%), yY/3O (36.7%), 3130 (20.0%) and 23130 (78.7%), respectively on light microscopy. Estrogen receptor (ER) immunoreactivity was not observed in any of the 30 cases examined. Normal gastric nomcosu was negative /o, P'4S0u,unx and P-4S0suc. Examination of serial sections revealed that imnxmmomactinityofE 2amd P'4SOo,om were located in the same cells of cu,cinonxotous glands. Imnxunoelectrun microscopy demonstrated that E 2 and }4SOurom were located along the membrane and ciste,nao of smooth endoplasmic reticulum(sER).Western blot analysis showed major band mfSS kDa of P-4SOa,arom in the gastric carcinoma tissues examined. Retrospective analysis of immunohistochemistry of E 2 in 208 cases of gastric carcinoma revealed that E 2 positive carcinoma cases were likely to demonstrate better survival rate than negative cases. These results above strongly suggest that E 2 is produced 6yoe xonmexpressed a,ovxatasu/mgastric carcinoma cells and is possibly involved /n the biology of gastric carcinoma cells.

Original languageEnglish
Pages (from-to)53-60
Number of pages8
JournalPathology Research and Practice
Volume188
Issue number1-2
DOIs
Publication statusPublished - 1992 Jan 1

Keywords

  • Aromatase
  • Estrogen receptor
  • Gastric carcinoma
  • Steroidogenesis estrogen

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology

Fingerprint Dive into the research topics of 'De novo expression of aromatase in gastric carcinoma: Light and electron microscopic immunohistochemical and Immunoblot study'. Together they form a unique fingerprint.

Cite this