Dark-field X-ray ptychography: Towards high-resolution imaging of thick and unstained biological specimens

Akihiro Suzuki, Kei Shimomura, Makoto Hirose, Nicolas Burdet, Yukio Takahashi

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

The phase shift of light or electrons in objects is now necessary for probing weak-phase objects such as unstained biological specimens. Optical microscopy (OM) and transmission electron microscopy (TEM) have been used to observe weak-phase objects. However, conventional OM has low spatial resolution and TEM is limited to thin specimens. Here, we report on the development of dark-field X-ray ptychography, which combines X-ray ptychography and X-ray in-line holography, to observe weak-phase objects with a phase resolution better than 0.01 rad, a spatial resolution better than 15 nm, and a field of view larger than 5 μm. We apply this method to the observation of both the outline and magnetosomes of the magnetotactic bacteria MO-1. Observation of thick samples with high resolution is expected to find broad applications in not only biology but also materials science.

Original languageEnglish
Article number35060
JournalScientific reports
Volume6
DOIs
Publication statusPublished - 2016 Oct 13
Externally publishedYes

ASJC Scopus subject areas

  • General

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