DAP10 associates with Ly49 receptors but contributes minimally to their expression and function in vivo

Ilaria Tassi, Gaelle Le Friec, Susan Gilfillan, Toshiyuki Takai, Wayne M. Yokohama, Marco Colonna

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

NK cells recognize target cells through activating receptors, many of which rely on the transmembrane adaptors DAP10, DAP12 and FcR-γ to deliver intracellular signals. Because these adaptors initiate distinct signaling pathways, they dictate the type of response mediated by receptor engagement. DAP10, for example, primarily triggers cytotoxicity, whereas DAP12 induces both cytotoxicity and IFN-γ secretion. In mice, NKG2D signals through both DAP10 and DAP12, which broadens and modulates the type of response engendered by encounter with ligand. Although initial studies indicated that Ly49H and Ly49D recruit only DAP12, a recent report suggested that they also associate with DAP10. We asked whether this association occurs and is functionally significant under physiologic conditions. Our data demonstrate that DAP10 does associate with Ly49H and Ly49D in primary NK cells. While this association contributes slightly to cell surface expression of both receptors, it has no significant impact on Ly49H-mediated control of murine cytomegalovirus infection. Thus, while many activating NK-cell receptors are promiscuous in terms of adaptor association, our data indicate that the functional consequences of such promiscuity may vary widely and may not be evident in all cases.

Original languageEnglish
Pages (from-to)1129-1135
Number of pages7
JournalEuropean Journal of Immunology
Volume39
Issue number4
DOIs
Publication statusPublished - 2009 Jun 26

Keywords

  • Cytomegalovirus
  • Innate immunity
  • Signaling

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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