Cytotoxicity and antigenicity of antimicrobial synthesized peptides derived from the beetle Allomyrina dichotoma defensin in mice

Yukari Koyama, Maki Motobu, Kenji Hikosaka, Manabu Yamada, Kikuyasu Nakamura, Hisako Saido-Sakanaka, Ai Asaoka, Minoru Yamakawa, Takashi Isobe, Kameo Shimura, Chung Boo Kang, Hideki Hayashidani, Yutaka Nakai, Yoshikazu Hirota

    Research output: Contribution to journalArticlepeer-review

    9 Citations (Scopus)

    Abstract

    Synthetic peptides, peptides A (Arg-Leu-Tyr-Leu-Arg-Ile-Gly-Arg-Arg-NH2) and B (Arg-Leu-Arg-Leu-Arg-Ile-Gly-Arg-Arg-NH2), derived from the beetle Allomyrina dichotoma defensin, have antimicrobial activities. Immunotoxicological effect of these peptides was evaluated by cytotoxicity of mouse peritoneal macrophages. In addition, antigenicity of these peptides was studied by evaluating antibody responses in mice immunized with these peptides. The toxicity of peptide A toward mouse peritoneal cells was less than that of polymyxin B, when morphologically evaluated in a cytotoxicity test. Almost all of mice injected intraperitoneally (i.p.) with either peptide A or B at 50-150 mg/kg survived, whereas all mice injected i.p. with polymyxin B at the doses of more than 25 mg/kg died within 24 h. Interestingly, almost all of mice injected intravenously with these peptides at the doses of 10 and 25 mg/kg also survived. Furthermore, mice immunized with these peptides conjugated with keyhole limpet hemocyanin (KLH) showed little or negligible anti-peptide A or B antibody production, although anti-KLH antibody was significantly produced. The results indicated that peptides A and B were less cytotoxic than polymyxin B and also had poor antigenicity to produce specific antibody in mice.

    Original languageEnglish
    Pages (from-to)1748-1753
    Number of pages6
    JournalInternational Immunopharmacology
    Volume6
    Issue number11
    DOIs
    Publication statusPublished - 2006 Nov

    Keywords

    • Antimicrobial peptides
    • Cytotoxicity
    • Defensin
    • Polymyxin B
    • Specific antibody generation

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Immunology
    • Pharmacology

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