Cytotoxicity analysis of staphylococcal bi-component β-pore forming toxins using the CHO cells expressing human lymphocyte receptor CCR5

Zhao Peng, Nao Shibata, Hideaki Tada, Jun Kaneko

Research output: Contribution to journalArticlepeer-review

Abstract

CCR5-mediated cytotoxicity of staphylococcal bi-component toxins was investigated using human CCR5-expressing CHO cells. Cytotoxicity of rim domain loop-exchange mutants between LukE and Hlg2 indicated that loop-4 of LukE is essential for cytotoxicity in combination with LukD. Interestingly, Hlg2 showed LukF-dependent CCR5-mediated cytotoxicity, suggesting that the F-components of toxins also play a role in the cell-specific cytotoxicity.

Original languageEnglish
Pages (from-to)2094-2097
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume82
Issue number12
DOIs
Publication statusPublished - 2018

Keywords

  • CCR5
  • Cytotoxicity
  • Staphylococcal β-PFTs

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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