Cytoskeleton dynamics control the first asymmetric cell division in Arabidopsis zygote

Yusuke Kimata, Takumi Higaki, Tomokazu Kawashima, Daisuke Kurihara, Yoshikatsu Sato, Tomomi Yamada, Seiichiro Hasezawa, Frederic Berger, Tetsuya Higashiyama, Minako Ueda

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

The asymmetric cell division of the zygote is the initial and crucial developmental step in most multicellular organisms. In flowering plants, whether zygote polarity is inherited from the preexisting organization in the egg cell or reestablished after fertilization has remained elusive. How dynamically the intracellular organization is generated during zygote polarization is also unknown. Here, we used a live-cell imaging system with Arabidopsis zygotes to visualize the dynamics of the major elements of the cytoskeleton, microtubules (MTs), and actin filaments (F-actins), during the entire process of zygote polarization. By combining image analysis and pharmacological experiments using specific inhibitors of the cytoskeleton, we found features related to zygote polarization. The preexisting alignment of MTs and F-actin in the egg cell is lost on fertilization. Then, MTs organize into a transverse ring defining the zygote subapical region and driving cell outgrowth in the apical direction. F-actin forms an apical cap and longitudinal arrays and is required to position the nucleus to the apical region of the zygote, setting the plane of the first asymmetrical division. Our findings show that, in flowering plants, the preexisting cytoskeletal patterns in the egg cell are lost on fertilization and that the zygote reorients the cytoskeletons to perform directional cell elongation and polar nuclear migration.

Original languageEnglish
Pages (from-to)14157-14162
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number49
DOIs
Publication statusPublished - 2016 Dec 6
Externally publishedYes

Keywords

  • Actin filament
  • Apical-basal axis
  • Arabidopsis thaliana
  • Microtubule
  • Zygote polarity

ASJC Scopus subject areas

  • General

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