Cytoplasmicnuclear genomic barriers in rice pollen development revealed by comparison of global gene expression profiles among five independent cytoplasmic male sterile lines

Sota Fujii, Mari Yamada, Masahiro Fujita, Etsuko Itabashi, Kazuki Hamada, Kentaro Yano, Nori Kurata, Kinya Toriyama

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Cytoplasmic male sterility (CMS) is one of the most ideal phenomena known in higher plants to describe the incompatibilities between mitochondrialnuclear genomic interactions. To elucidate the dependency of pollen development on mitochondrial genotypes and cytoplasmicnuclear genomic barriers, we employed five CMS isogenic lines of rice, CW-, W11-, LD-, BT-and WA-type CMS lines, that exhibit distinct pollen-defective phenotypes, and we characterized the CMS phenotypes and the nuclear gene expression patterns in conjunction with their mitochondrial genomic structures. These five CMS lines carried independent mitotypes, and W11, LD and BT mitochondrial genomes were relatively close with respect to their phylogeny. In anthers at the uninucleate microspore and bicellular pollen stages, 8,199 genes significantly changed their expression in at least one of the CMS lines. Common expression patterns were observed in BT, LD and W11 after k-means clustering. Among the genes encoding putative mitochondrial proteins, ALTERNATIVE OXIDASE 1A, a gene for the well-known mitochondrial stress marker, was included in the group ectopically up-regulated in anthers at the bicellular pollen stage of BT, LD and W11. Several other clusters were also regulated in a cytoplasm-specific manner during pollen development. These clear similarities in gene regulatory networks of BT-, LD-and W11-CMS lines indicate that the phylogenetic relationships of the mitochondrial genotypes are strongly correlated with nuclear gene expression patterns and pollen abortion phenotypes, providing evidence of the mitochondrial epistacy over the nuclear genome during pollen development.

Original languageEnglish
Pages (from-to)610-620
Number of pages11
JournalPlant and Cell Physiology
Volume51
Issue number4
DOIs
Publication statusPublished - 2010 Apr 1

Keywords

  • Cytoplasmic male sterility
  • Epistacy
  • Microarray
  • Mitochondria
  • Oryza sativa L
  • Pollen development

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

Fingerprint Dive into the research topics of 'Cytoplasmicnuclear genomic barriers in rice pollen development revealed by comparison of global gene expression profiles among five independent cytoplasmic male sterile lines'. Together they form a unique fingerprint.

  • Cite this