Cytoplasmic motility reflects phagocytic activity in alveolar macrophages from dog lungs

Mutsuo Yamaya, Takeyasu Fukushima, Kiyohisa Sekizawa, Takashi Ohrui, Hidetada Sasaki

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

To determine whether cytoplasmic motility relates to phagocytic activity of alveolar macrophages (AM), we measured the remanent field strength (RFS) from the AM containing Fe3O4 particles (5 × 106 cells) and the number of phagocytized fluorescent latex spheres in the AM without having Fe3O4 particles (106 cells) harvested by broncho-alveolar lavage from dog lungs in vitro. Cytoplasmic motility was estimated from the relaxation rate (λo; min-1) calculated from the decay curve of RFS and the number of phagocytized latex spheres was counted using fluorescent microscopy after the addition of latex spheres (5 × 107). The tumor necrosis factor increased both λo and the number of phagocytized latex spheres, and cytochalasin D and colchicine decreased them in a concentration-dependent fashion. Increases and decreases in λo induced by drugs paralleled the number of phagocytized latex spheres. These results suggest that cytoplasmic motility reflects phagocytic activity of AM and both cytoplasmic movement and phagocytosis may be regulated by a similar mechanism in the cytoskeletal system.

Original languageEnglish
Pages (from-to)199-205
Number of pages7
JournalRespiration Physiology
Volume101
Issue number2
DOIs
Publication statusPublished - 1995 Aug

Keywords

  • Alveolar macrophages
  • Cytoplasmic motility
  • Cytoskeleton
  • Macrophages (Alveolar)
  • Mammals (Dog)

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine

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