TY - JOUR
T1 - Cytoplasmic motility reflects phagocytic activity in alveolar macrophages from dog lungs
AU - Yamaya, Mutsuo
AU - Fukushima, Takeyasu
AU - Sekizawa, Kiyohisa
AU - Ohrui, Takashi
AU - Sasaki, Hidetada
N1 - Funding Information:
We thank Mr. Grant Crittenden for correcting the English. This study was supported by SRF Grant for Biomedical Research.
PY - 1995/8
Y1 - 1995/8
N2 - To determine whether cytoplasmic motility relates to phagocytic activity of alveolar macrophages (AM), we measured the remanent field strength (RFS) from the AM containing Fe3O4 particles (5 × 106 cells) and the number of phagocytized fluorescent latex spheres in the AM without having Fe3O4 particles (106 cells) harvested by broncho-alveolar lavage from dog lungs in vitro. Cytoplasmic motility was estimated from the relaxation rate (λo; min-1) calculated from the decay curve of RFS and the number of phagocytized latex spheres was counted using fluorescent microscopy after the addition of latex spheres (5 × 107). The tumor necrosis factor increased both λo and the number of phagocytized latex spheres, and cytochalasin D and colchicine decreased them in a concentration-dependent fashion. Increases and decreases in λo induced by drugs paralleled the number of phagocytized latex spheres. These results suggest that cytoplasmic motility reflects phagocytic activity of AM and both cytoplasmic movement and phagocytosis may be regulated by a similar mechanism in the cytoskeletal system.
AB - To determine whether cytoplasmic motility relates to phagocytic activity of alveolar macrophages (AM), we measured the remanent field strength (RFS) from the AM containing Fe3O4 particles (5 × 106 cells) and the number of phagocytized fluorescent latex spheres in the AM without having Fe3O4 particles (106 cells) harvested by broncho-alveolar lavage from dog lungs in vitro. Cytoplasmic motility was estimated from the relaxation rate (λo; min-1) calculated from the decay curve of RFS and the number of phagocytized latex spheres was counted using fluorescent microscopy after the addition of latex spheres (5 × 107). The tumor necrosis factor increased both λo and the number of phagocytized latex spheres, and cytochalasin D and colchicine decreased them in a concentration-dependent fashion. Increases and decreases in λo induced by drugs paralleled the number of phagocytized latex spheres. These results suggest that cytoplasmic motility reflects phagocytic activity of AM and both cytoplasmic movement and phagocytosis may be regulated by a similar mechanism in the cytoskeletal system.
KW - Alveolar macrophages
KW - Cytoplasmic motility
KW - Cytoskeleton
KW - Macrophages (Alveolar)
KW - Mammals (Dog)
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U2 - 10.1016/0034-5687(95)00019-A
DO - 10.1016/0034-5687(95)00019-A
M3 - Article
C2 - 8570922
AN - SCOPUS:0029131037
VL - 101
SP - 199
EP - 205
JO - Respiratory Physiology and Neurobiology
JF - Respiratory Physiology and Neurobiology
SN - 1569-9048
IS - 2
ER -