Cytoplasmic male sterility-associated ORF79 is toxic to plant regeneration when expressed with mitochondrial targeting sequence of ATPase γ subunit

Hisayo Kojima, Tomohiko Kazama, Sota Fujii, Kinya Toriyama

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Cytoplasmic male sterility (CMS) is a maternally inherited trait that results in the inability of plants to produce functional pollen. CMS is considered as a phenomenon caused by aberrant mitochondrial genomes. In BT-type CMS of rice (Oryza sativa L.), a CMS-associated mitochondrial gene has been reported to be an orf79 gene. To confirm the effect of ORF79 on cell fate, we introduced the orf79 gene with or without a mitochondrial targeting sequence into Taichung 65 carrying normal cytoplasm. It was revealed that ORF79 was toxic to plant regeneration when expressed as a fusion with mitochondrial targeting sequence of the ATPase γ subunit. This result implicates ORF79 as cytotoxic, and its toxicity depends on its combination with a mitochondrial targeting sequence.

Original languageEnglish
Pages (from-to)111-114
Number of pages4
JournalPlant Biotechnology
Volume27
Issue number1
DOIs
Publication statusPublished - 2010

Keywords

  • Cytoplasmic male sterility
  • Mitochondria
  • Orf79
  • Oryza sativa L

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

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