Cytologic application of p53 overexpression using immunocytochemistry and confocal laser scanning microscopy

Shinji Sato, Kiyoshi Ito, Kumiko Jiko, Ryo Konno, Akira Yajima, Hironobu Sasano

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

OBJECTIVE: p53 Protein plays an important role in cellular growth control. This study investigated p53 protein expression in cell smears of endometrial carcinomas supplemented by confocal laser scanning microscopy (CLSM). STUDY DESIGN: Imprints from surgical specimens of 20 endometrial carcinomas were used. p53 Protein expression was investigated immunocytochemically using the monoclonal antibody pAb1801. Using CLSM, three-dimensional morphology was studied. RESULTS: Of the 20 cases of endometrial carcinoma, 8 stained positively for p53 protein. p53 Showed heterogeneous intranuclear localization, which appeared to be associated with chromatin structure. CONCLUSION: Immunocytochemical detection of p53 overexpression in cell samples is practical, and CLSM has vast potentials in studying the intranuclear arrangement of chromatin.

Original languageEnglish
Pages (from-to)342-345
Number of pages4
JournalActa Cytologica
Volume42
Issue number2
DOIs
Publication statusPublished - 1998

Keywords

  • Immunocytochemistry
  • Laser scanning confocal microscopy
  • p53 protein

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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