Cytokinin-induced expression of OSH1 in a shoot-regenerating rice callus

Masashi Naruse; Honami Takahashi; Nori Kurata; Yukihiro Ito

doi:10.5511/plantbiotechnology.18.0614a

Cytokinin-induced expression of OSH1 in a shoot-regenerating rice callus

Masashi Naruse, Honami Takahashi, Nori Kurata, Yukihiro Ito

AGR - Life Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

The expression of a KNOX class 1 gene OSH1 is induced by cytokinin during regeneration of shoots from callus in Oryza sativa L. (rice). This cytokinin-induced expression was enhanced by overexpression of homologues of cytokinin-signalling phosphorelay genes such as a histidine kinase gene OHK3, a phosphotransmitter gene OHP2 and a response regulator gene ORR1 in cultured cells. Regionally overlapped expression of these genes and OSH1 was observed in shoot apex. These results suggest that these cytokinin-signalling genes are positive regulators of the expression of OSH1, and mediate the OSH expression upon shoot regeneration from callus in rice.

Original language	English
Pages (from-to)	267-272
Number of pages	6
Journal	Plant Biotechnology
Volume	35
Issue number	3
DOIs	https://doi.org/10.5511/plantbiotechnology.18.0614a
Publication status	Published - 2018

Keywords

Cytokinin
KNOX gene
Rice
Shoot regeneration

ASJC Scopus subject areas

Biotechnology
Agronomy and Crop Science
Plant Science

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10.5511/plantbiotechnology.18.0614a

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@article{71334417b45a4d3bbf8b73af672c549d,

title = "Cytokinin-induced expression of OSH1 in a shoot-regenerating rice callus",

abstract = "The expression of a KNOX class 1 gene OSH1 is induced by cytokinin during regeneration of shoots from callus in Oryza sativa L. (rice). This cytokinin-induced expression was enhanced by overexpression of homologues of cytokinin-signalling phosphorelay genes such as a histidine kinase gene OHK3, a phosphotransmitter gene OHP2 and a response regulator gene ORR1 in cultured cells. Regionally overlapped expression of these genes and OSH1 was observed in shoot apex. These results suggest that these cytokinin-signalling genes are positive regulators of the expression of OSH1, and mediate the OSH expression upon shoot regeneration from callus in rice.",

keywords = "Cytokinin, KNOX gene, Rice, Shoot regeneration",

author = "Masashi Naruse and Honami Takahashi and Nori Kurata and Yukihiro Ito",

note = "Funding Information: Full-length cDNAs were developed by the Rice Genome Project of the National Institute of Agrobiological Sciences, Japan, and were provided by the Rice Genome Resource Center. We thank Tomomi Makino, Satomi Sakai, Akemi Ishii and Yoko Shiroto for their technical assistance. This work was supported in part by Kuribayashi Education and Science foundation. Publisher Copyright: {\textcopyright} 2018 The Japanese Society for Plant Cell and Molecular Biology.",

year = "2018",

doi = "10.5511/plantbiotechnology.18.0614a",

language = "English",

volume = "35",

pages = "267--272",

journal = "Plant Biotechnology",

issn = "1342-4580",

publisher = "Japanese Society for Plant Cell and Molecular Biology",

number = "3",

}

TY - JOUR

T1 - Cytokinin-induced expression of OSH1 in a shoot-regenerating rice callus

AU - Naruse, Masashi

AU - Takahashi, Honami

AU - Kurata, Nori

AU - Ito, Yukihiro

N1 - Funding Information: Full-length cDNAs were developed by the Rice Genome Project of the National Institute of Agrobiological Sciences, Japan, and were provided by the Rice Genome Resource Center. We thank Tomomi Makino, Satomi Sakai, Akemi Ishii and Yoko Shiroto for their technical assistance. This work was supported in part by Kuribayashi Education and Science foundation. Publisher Copyright: © 2018 The Japanese Society for Plant Cell and Molecular Biology.

PY - 2018

Y1 - 2018

N2 - The expression of a KNOX class 1 gene OSH1 is induced by cytokinin during regeneration of shoots from callus in Oryza sativa L. (rice). This cytokinin-induced expression was enhanced by overexpression of homologues of cytokinin-signalling phosphorelay genes such as a histidine kinase gene OHK3, a phosphotransmitter gene OHP2 and a response regulator gene ORR1 in cultured cells. Regionally overlapped expression of these genes and OSH1 was observed in shoot apex. These results suggest that these cytokinin-signalling genes are positive regulators of the expression of OSH1, and mediate the OSH expression upon shoot regeneration from callus in rice.

AB - The expression of a KNOX class 1 gene OSH1 is induced by cytokinin during regeneration of shoots from callus in Oryza sativa L. (rice). This cytokinin-induced expression was enhanced by overexpression of homologues of cytokinin-signalling phosphorelay genes such as a histidine kinase gene OHK3, a phosphotransmitter gene OHP2 and a response regulator gene ORR1 in cultured cells. Regionally overlapped expression of these genes and OSH1 was observed in shoot apex. These results suggest that these cytokinin-signalling genes are positive regulators of the expression of OSH1, and mediate the OSH expression upon shoot regeneration from callus in rice.

KW - Cytokinin

KW - KNOX gene

KW - Rice

KW - Shoot regeneration

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DO - 10.5511/plantbiotechnology.18.0614a

M3 - Article

AN - SCOPUS:85056296283

VL - 35

SP - 267

EP - 272

JO - Plant Biotechnology

JF - Plant Biotechnology

SN - 1342-4580

IS - 3

ER -

Cytokinin-induced expression of OSH1 in a shoot-regenerating rice callus

Abstract

Keywords

ASJC Scopus subject areas

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