Cytokine-mediated xanthine oxidase upregulation in chronic obstructive pulmonary disease's airways

Yuichi Komaki, Hisatoshi Sugiura, Akira Koarai, Masafumi Tomaki, Hiromasa Ogawa, Takefumi Akita, Toshio Hattori, Masakazu Ichinose

Research output: Contribution to journalArticlepeer-review

52 Citations (Scopus)


Reactive oxygen species have been reported to be involved in the airway inflammatory process of chronic obstructive pulmonary disease (COPD). The aim of this study was to quantify the activity of xanthine oxidase (XO), which generates a potent radical superoxide anion in COPD airways. Thirteen stable COPD patients and 10 healthy subjects participated in this study. We collected the epithelial lining fluid using a newly developed microsampling technique, and quantified of cytokines responsible for the XO gene upregulation. The XO activity was significantly increased in COPD patients compared with that in healthy subjects. A significant negative correlation was found between the XO activity and the %FEV1 values. The level of tumor necrosis factor-α, interleukin-1β, and interferon-γ in COPD patients was significantly higher than that in healthy subjects. Both the amount of tumor necrosis factor-α and interleukin-1β were significantly correlated with the degree of XO activity. These results suggest that the XO activity is increased in COPD airways, possibly due to its gene upregulation by proinflammatory cytokines. Because the XO activity was significantly correlated with the degree of airway obstruction, these cytokine-XO production pathways may play a key role in the inflammation of COPD.

Original languageEnglish
Pages (from-to)297-302
Number of pages6
JournalPulmonary Pharmacology and Therapeutics
Issue number4
Publication statusPublished - 2005 Aug


  • IFN-γ
  • IL-1β
  • Oxidative stress
  • Superoxide
  • TNF-α

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Biochemistry, medical
  • Pharmacology (medical)


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