Cytokine induction by extracellular products of oral viridans group streptococci

H. Takada, Y. Kawabata, M. Tamura, K. Matsushita, H. Igarashi, H. Ohkuni, Y. Todome, T. Uchiyama, S. Kotani

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)


During an etiological study of Kawasaki disease (mucocutaneous lymph node syndrome [MCLS]), we found that dominant viridans streptococcal strains on tooth surfaces and in the throat of both MCLS patients and non-MCLS control children formed erythrogenic and biologically active, extracellular products. In this study, we demonstrated that erythrogenic culture supernatant concentrates of representative strains (two Streptococcus mitis and two Streptococcus oralis), when injected intravenously, induced serum tumor necrosis factor alpha, interleukin-6 (IL-6), and gamma interferon in muramyldipeptide- or Propionibacterium acnes-primed C3H/HeN mice. The concentrates also induced tumor necrosis factor alpha, IL-6, and thymocyte- activating factor (essentially IL-1) in murine peritoneal macrophage, human monocyte, and human whole-blood cultures. An erythrogenic, heat-labile extracellular protein fraction (F-1) that was concentrated from the culture supernatants of a representative S. mitis strain exhibited the above- mentioned cytokine-inducing activity. This partially purified F-1 fraction also induced thymocyte-activating factor and IL-6 in human umbilical vascular endothelial cell and gingival fibroblast cultures.

Original languageEnglish
Pages (from-to)5252-5260
Number of pages9
JournalInfection and immunity
Issue number12
Publication statusPublished - 1993 Dec 14

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases


Dive into the research topics of 'Cytokine induction by extracellular products of oral viridans group streptococci'. Together they form a unique fingerprint.

Cite this