TY - JOUR
T1 - Cytokine-induced fungicidal activity of human polymorphonuclear leukocytes against Penicillium marneffei
AU - Kudeken, Norifumi
AU - Kawakami, Kazuyoshi
AU - Saito, Atsushi
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid for Scientific Research (C) (09670292) from the Ministry of Education, Science, Sport and Culture, by grants from the Ministry of Health and Welfare, Japan and by the Japan Health Science Foundation. The authors thank Dr. F.G. Issa (Department of Medicine, University of Sydney, Sydney, Australia) for editing the manuscript.
PY - 1999/11
Y1 - 1999/11
N2 - We investigated the fungicidal activity of human polymorphonuclear leukocytes (PMN) against Penicillium marneffei. The yeast cells were cocultured in vitro with PMN for 24 h. Microscopic examination was also performed to examine the germination of yeast cells and their transformation to hyphal form during culture. Unstimulated PMN inhibited fungal growth when used at a higher effector/target (E/T) ratio but inhibited germination at a lower E/T ratio. We also examined the effects of various PMN-activating cytokines, including granulocyte-macrophage colony stimulating factor (GM-CSF), G-CSF, interleukin (IL)-8, interferon (IFN)-γ and tumor necrosis factor (TNF)-α on the activity of PMN. Among these, GM-CSF, G-CSF and IFN-γ enhanced PMN activity from being fungistatic to fungicidal. However, the other cytokines had little or no effect on PMN activity. In contrast, all tested cytokines enhanced PMN inhibitory effects on germination and morphological changes of P. marneffei. These antifungal activities were most strongly induced by GM-CSF. The combined use of any of the above cytokines failed to synergistically enhance antifungal PMN activity. Our results demonstrated that cytokine-activated PMN exert a significant antifungal activity, by suppressing the growth and germination of P. marneffei. Our results suggest that PMN may contribute to host resistance to infection against this fungal pathogen. Copyright (C) 1999 Federation of European Microbiological Societies.
AB - We investigated the fungicidal activity of human polymorphonuclear leukocytes (PMN) against Penicillium marneffei. The yeast cells were cocultured in vitro with PMN for 24 h. Microscopic examination was also performed to examine the germination of yeast cells and their transformation to hyphal form during culture. Unstimulated PMN inhibited fungal growth when used at a higher effector/target (E/T) ratio but inhibited germination at a lower E/T ratio. We also examined the effects of various PMN-activating cytokines, including granulocyte-macrophage colony stimulating factor (GM-CSF), G-CSF, interleukin (IL)-8, interferon (IFN)-γ and tumor necrosis factor (TNF)-α on the activity of PMN. Among these, GM-CSF, G-CSF and IFN-γ enhanced PMN activity from being fungistatic to fungicidal. However, the other cytokines had little or no effect on PMN activity. In contrast, all tested cytokines enhanced PMN inhibitory effects on germination and morphological changes of P. marneffei. These antifungal activities were most strongly induced by GM-CSF. The combined use of any of the above cytokines failed to synergistically enhance antifungal PMN activity. Our results demonstrated that cytokine-activated PMN exert a significant antifungal activity, by suppressing the growth and germination of P. marneffei. Our results suggest that PMN may contribute to host resistance to infection against this fungal pathogen. Copyright (C) 1999 Federation of European Microbiological Societies.
KW - Cytokine
KW - Human
KW - PMN
KW - Penicilliosis
KW - Penicillium marneffei
KW - Yeast
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U2 - 10.1016/S0928-8244(99)00128-5
DO - 10.1016/S0928-8244(99)00128-5
M3 - Article
C2 - 10536297
AN - SCOPUS:0032833912
VL - 26
SP - 115
EP - 124
JO - Pathogens and Disease
JF - Pathogens and Disease
SN - 2049-632X
IS - 2
ER -
