Cyclin-D1-gene amplification is a more potent prognostic factor than its protein over-expression in human head-and-neck squamous-cell carcinoma

Ryoichi Kyomoto, Hirobumi Kumazawa, Yoshinobu Toda, Noriko Sakaida, Akiharu Okamura, Michitaka Iwanaga, Masayuki Shintaku, Toshio Yamashita, Hiroshi Hiai, Manabu Fukumoto

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

To evaluate the prognostic significance of cyclin D1 protein/gene expressions in human head-and-neck squamous-cell carcinoma (HNSCC), we examined amplification of the cyclin-D1 gene (CCND1) by the differential PCR method and over-expression of cyclin-D1 protein by immunohistochemistry in 45 paraffin-embedded sections from HNSCC. Amplification of CCND1 was found in 10 (22%) cases and overexpression of cyclin D1 was found in 24 (53%) cases. CCND1 amplification was also found in 3 (25%) of 12 cases of dysplastic lesions adjacent to HNSCC. The overall 5-year survival of patients with CCND1 amplification or with protein over-production was significantly lower than that of patients without (p < 0.0001 and p < 0.05, respectively). However, with multivariate analysis, only amplification of CCND1 retained an independent prognostic value (p = 0.0018). These suggest that CCND1 amplification occurs at early stages of HNSCC tumorigenesis and is a more useful prognostic factor than over-expression of cyclin D1 in HNSCC.

Original languageEnglish
Pages (from-to)576-581
Number of pages6
JournalInternational Journal of Cancer
Volume74
Issue number6
DOIs
Publication statusPublished - 1997 Dec 1

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Cyclin-D1-gene amplification is a more potent prognostic factor than its protein over-expression in human head-and-neck squamous-cell carcinoma'. Together they form a unique fingerprint.

  • Cite this