Cyclical tensile force on periodontal ligament cells inhibits osteoclastogenesis through OPG induction

Hiroyuki Kanzaki, M. Chiba, A. Sato, A. Miyagawa, K. Arai, S. Nukatsuka, H. Mitani

Research output: Contribution to journalArticlepeer-review

71 Citations (Scopus)


The periodontal ligament (PDL) maintains homeostasis of periodontal tissue under mechanical tensile-loading caused by mastication. Occlusal load inhibits atrophic alveolar bone resorption. Previously, we discovered that continuous compressive force on PDL cells induced osteoclastogenesis-supporting activity, with up-regulation of RANKL. We hypothesized that, unlike compression, cyclical tensile force upregulates OPG expression in PDL cells via TGF-beta up-regulation, and does not induce osteoclastogenesis-supporting activity. PDL cells were mechanically stimulated by cyclical tensile force in vitro. The conditioned media of PDL cells that had been subjected to cyclical tensile force inhibited osteoclastogenesis. Cyclical tensile force up-regulated not only RANKL mRNA expression, but also OPG mRNA expression in PDL cells. Tensile force up-regulated TGF-beta expression in PDL cells as well. Administration of neutralizing antibodies to TGF-beta inhibited OPG upregulation under cyclical tensile-force stimulation in a dose-dependent manner. Additionally, the osteoclastogenesis-inhibitory effect of the conditioned media of PDL cells under cyclical tensile force was partially rescued by the administration of TGF-beta neutralizing antibodies. In conclusion, tensile force inhibited the osteoclastogenesis-supporting activity of PDL cells by inducing the up-regulation of OPG via TGF-beta stimulation.

Original languageEnglish
Pages (from-to)457-462
Number of pages6
JournalJournal of dental research
Issue number5
Publication statusPublished - 2006 May


  • Occlusal force
  • Osteoclastogenesis
  • Osteoprotegerin (OPG)
  • TGF-beta
  • Tensile force

ASJC Scopus subject areas

  • Dentistry(all)


Dive into the research topics of 'Cyclical tensile force on periodontal ligament cells inhibits osteoclastogenesis through OPG induction'. Together they form a unique fingerprint.

Cite this