Cyclic sample pooling using two-dimensional liquid chromatography system enhances coverage in shotgun proteomics

Yusuke Kawashima, Mamoru Satoh, Tatsuya Saito, Takashi Matsui, Fumio Nomura, Hiroyuki Matsumoto, Yoshio Kodera

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)

    Abstract

    We report a cyclic sample pooling technique devised in two-dimensional liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) shotgun proteomics that renders deeper proteome coverage; we combined low pH reversed-phase (RP) LC in trifluoroacetic acid in the first dimension, followed by cyclic sample pooling of the eluate and low-pH RP-LC in formic acid in the second dimension. The new protocol has a significantly higher resolving power suitable for LC-ESI-MS/MS shotgun proteomics.

    Original languageEnglish
    Pages (from-to)691-694
    Number of pages4
    JournalBiomedical Chromatography
    Volume27
    Issue number6
    DOIs
    Publication statusPublished - 2013 Jun

    Keywords

    • 2D-LC-ESI-MS
    • Cyclic sample pooling
    • Serum proteins
    • Shotgun proteomics

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Biochemistry
    • Molecular Biology
    • Pharmacology
    • Drug Discovery
    • Clinical Biochemistry

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