TY - JOUR
T1 - Cyclic nucleotide-gated ion channel-mediated cell death may not be critical for R gene-conferred resistance to Cucumber mosaic virus in Arabidopsis thaliana
AU - Takahashi, Hideki
AU - Kai, Asako
AU - Yamashita, Masao
AU - Ando, Sugihiro
AU - Sekine, Ken Taro
AU - Kanayama, Yoshinori
AU - Tomita, Hirofumi
N1 - Funding Information:
This research was supported in part by a grant-in-aid for Scientific Research (B) ( 22380028 ) from the Ministry of Education, Culture, Sports and Arts, Japan . We thank the Arabidopsis Biological Resource Center (ABRC) at Ohio State University, Columbus, OH, USA for kindly providing dnd1 seed. We also thank to Ms. Kazumi Haga for her technical assistance.
PY - 2012/7
Y1 - 2012/7
N2 - Virus multiplication is dependent on host metabolism. Thus, cell death at the primary site of virus infection seems to be critical for defense against virus infection. However, the importance of cell death in hypersensitive response (HR) resistance against viruses in host plants is still unclear. To investigate the role of cell death in virus resistance, we analyzed the response of the . Arabidopsis defense, no death (. dnd) mutant to . Cucumber mosaic virus (CMV) in the presence of . RCY1 encoding a CC-NB-LRR class R-protein in the background of ecotype Col-0. The . dnd1 phenotype, loss of HR cell death without loss of gene-for-gene resistance to bacterial pathogens, is caused by mutation of the gene encoding the cyclic nucleotide-gated ion channel . AtCNGC2. In virus-inoculated leaves of the . dnd1 mutant carrying . RCY1 (. RCY1 dnd1), the induction of HR cell death at the site of virus infection was significantly compromised. However, like wild-type carrying . RCY1, local and systemic spread of the virus was restricted and expression of . pathogenesis-related (. PR) genes was induced in the . RCY1 dnd1 mutant. The response to CMV in the . RCY1 dnd1 mutant, which was transformed with a salicylic acid-inducible promoter P4::green fluorescence protein-coding region (. P4pro::GFP) construct, suggested that the salicylic acid signaling pathway was activated at the site of virus infection without induction of HR cell death. Thus, . DND1-mediated HR cell death may not play a critical role in . RCY1-conferred resistance to CMV in . Arabidopsis.
AB - Virus multiplication is dependent on host metabolism. Thus, cell death at the primary site of virus infection seems to be critical for defense against virus infection. However, the importance of cell death in hypersensitive response (HR) resistance against viruses in host plants is still unclear. To investigate the role of cell death in virus resistance, we analyzed the response of the . Arabidopsis defense, no death (. dnd) mutant to . Cucumber mosaic virus (CMV) in the presence of . RCY1 encoding a CC-NB-LRR class R-protein in the background of ecotype Col-0. The . dnd1 phenotype, loss of HR cell death without loss of gene-for-gene resistance to bacterial pathogens, is caused by mutation of the gene encoding the cyclic nucleotide-gated ion channel . AtCNGC2. In virus-inoculated leaves of the . dnd1 mutant carrying . RCY1 (. RCY1 dnd1), the induction of HR cell death at the site of virus infection was significantly compromised. However, like wild-type carrying . RCY1, local and systemic spread of the virus was restricted and expression of . pathogenesis-related (. PR) genes was induced in the . RCY1 dnd1 mutant. The response to CMV in the . RCY1 dnd1 mutant, which was transformed with a salicylic acid-inducible promoter P4::green fluorescence protein-coding region (. P4pro::GFP) construct, suggested that the salicylic acid signaling pathway was activated at the site of virus infection without induction of HR cell death. Thus, . DND1-mediated HR cell death may not play a critical role in . RCY1-conferred resistance to CMV in . Arabidopsis.
KW - Cell death
KW - Cucumber mosaic virus (CMV)
KW - Defense response
KW - Green fluorescent protein
KW - Hypersensitive response (HR)
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U2 - 10.1016/j.pmpp.2012.03.003
DO - 10.1016/j.pmpp.2012.03.003
M3 - Article
AN - SCOPUS:84859929117
VL - 79
SP - 40
EP - 48
JO - Physiological and Molecular Plant Pathology
JF - Physiological and Molecular Plant Pathology
SN - 0885-5765
ER -