Cyclic electron flow around photosystem i via chloroplast NAD(P)H dehydrogenase (NDH) complex performs a significant physiological role during photosynthesis and plant growth at low temperature in rice

Wataru Yamori, Naoki Sakata, Yuji Suzuki, Toshiharu Shikanai, Amane Makino

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

The role of NAD(P)H dehydrogenase (NDH)-dependent cyclic electron flow around photosystem I in photosynthetic regulation and plant growth at several temperatures was examined in rice (Oryza sativa) that is defective in CHLORORESPIRATORY REDUCTION 6 (CRR6), which is required for accumulation of sub-complex A of the chloroplast NDH complex (crr6). NdhK was not detected by Western blot analysis in crr6 mutants, resulting in lack of a transient post-illumination increase in chlorophyll fluorescence, and confirming that crr6 mutants lack NDH activity. When plants were grown at 28 or 35°C, all examined photosynthetic parameters, including the CO 2 assimilation rate and the electron transport rate around photosystems I and II, at each growth temperature at light intensities above growth light (i.e. 800 μmol photons m -2 sec -1), were similar between crr6 mutants and control plants. However, when plants were grown at 20°C, all the examined photosynthetic parameters were significantly lower in crr6 mutants than control plants, and this effect on photosynthesis caused a corresponding reduction in plant biomass. The F v/F m ratio was only slightly lower in crr6 mutants than in control plants after short-term strong light treatment at 20°C. However, after long-term acclimation to the low temperature, impairment of cyclic electron flow suppressed non-photochemical quenching and promoted reduction of the plastoquinone pool in crr6 mutants. Taken together, our experiments show that NDH-dependent cyclic electron flow plays a significant physiological role in rice during photosynthesis and plant growth at low temperature.

Original languageEnglish
Pages (from-to)966-976
Number of pages11
JournalPlant Journal
Volume68
Issue number6
DOIs
Publication statusPublished - 2011 Dec 1

Keywords

  • CO assimilation
  • NAD(P)H dehydrogenase
  • cyclic electron transport
  • light response
  • photosynthesis
  • temperature response

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

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