TY - JOUR
T1 - Cutting edge
T2 - Combined treatment of TNF-α and IFN-γ causes redistribution of junctional adhesion molecule in human endothelial cells
AU - Ozaki, Harunobu
AU - Ishii, Kenji
AU - Horiuchi, Hisanori
AU - Arai, Hidenori
AU - Kawamoto, Takahiro
AU - Okawa, Katsuya
AU - Iwamatsu, Akihiro
AU - Kita, Toru
PY - 1999/7/15
Y1 - 1999/7/15
N2 - Proinflammatory cytokines such as TNF-α and IFN-γ induce cell adhesion molecules in endothelial cells and promote transmigration of leukocytes across endothelial cells. However, when those two were administered together, leukocyte transmigration paradoxically decreased. We cloned a human and bovine homologue of the junctional adhesion molecule (JAM), a novel molecule at the tight junction, and examined the effects of proinflammatory cytokines on JAM in HUVECs. The combined treatment of TNF-α plus IFN-γ caused a disappearance of JAM from intercellular junctions. However, flow cytometry, cell ELISA, and subcellular fractionation analysis demonstrated that the amount of JAM was ant reduced. This suggested that JAM changed its distribution in response to proinflammatory cytokine. This redistribution or JAM might be involved in a decrease in transendothelial migration of leukocytes at inflammatory sites.
AB - Proinflammatory cytokines such as TNF-α and IFN-γ induce cell adhesion molecules in endothelial cells and promote transmigration of leukocytes across endothelial cells. However, when those two were administered together, leukocyte transmigration paradoxically decreased. We cloned a human and bovine homologue of the junctional adhesion molecule (JAM), a novel molecule at the tight junction, and examined the effects of proinflammatory cytokines on JAM in HUVECs. The combined treatment of TNF-α plus IFN-γ caused a disappearance of JAM from intercellular junctions. However, flow cytometry, cell ELISA, and subcellular fractionation analysis demonstrated that the amount of JAM was ant reduced. This suggested that JAM changed its distribution in response to proinflammatory cytokine. This redistribution or JAM might be involved in a decrease in transendothelial migration of leukocytes at inflammatory sites.
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M3 - Article
C2 - 10395639
AN - SCOPUS:0033565947
VL - 163
SP - 553
EP - 557
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 2
ER -