CURLED LATER1 encoding the largest subunit of the Elongator complex has a unique role in leaf development and meristem function in rice

Hikari Matsumoto, Yukiko Yasui, Yoshihiro Ohmori, Wakana Tanaka, Tetsuya Ishikawa, Hisataka Numa, Kenta Shirasawa, Yojiro Taniguchi, Junichi Tanaka, Yasuhiro Suzuki, Hiro Yuki Hirano

Research output: Contribution to journalArticlepeer-review

Abstract

The Elongator complex, which is conserved in eukaryotes, has multiple roles in diverse organisms. In Arabidopsis thaliana, Elongator is shown to be involved in development, hormone action and environmental responses. However, except for Arabidopsis, our knowledge of its function is poor in plants. In this study, we initially carried out a genetic analysis to characterize a rice mutant with narrow and curled leaves, termed curled later1 (cur1). The cur1 mutant displayed a heteroblastic change, whereby the mutant leaf phenotype appeared specifically at a later adult phase of vegetative development. The shoot apical meristem (SAM) was small and the leaf initiation rate was low, suggesting that the activity of the SAM seemed to be partially reduced in cur1. We then revealed that CUR1 encodes a yeast ELP1-like protein, the largest subunit of Elongator. Furthermore, disruption of OsELP3 encoding the catalytic subunit of Elongator resulted in phenotypes similar to those of cur1, including the timing of the appearance of mutant phenotypes. Thus, Elongator activity seems to be specifically required for leaf development at the late vegetative phase. Transcriptome analysis showed that genes involved in protein quality control were highly upregulated in the cur1 shoot apex at the later vegetative phase, suggesting the restoration of impaired proteins probably produced by partial defects in translational control due to the loss of function of Elongator. The differences in the mutant phenotype and gene expression profile between CUR1 and its Arabidopsis ortholog suggest that Elongator has evolved to play a unique role in rice development.

Original languageEnglish
Pages (from-to)351-364
Number of pages14
JournalPlant Journal
Volume104
Issue number2
DOIs
Publication statusPublished - 2020 Oct 1
Externally publishedYes

Keywords

  • bulliform cell
  • Elongator complex
  • heteroblasty
  • leaf development
  • protein quality control
  • rice (Oryza sativa)
  • sclerenchyma
  • shoot apical meristem

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

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