Curcumin induces cross-regulation between autophagy and apoptosis in uterine leiomyosarcoma cells

Bin Li, Takashi Takeda, Kenji Tsuiji, Tze Fang Wong, Mari Tadakawa, Akiko Kondo, Satoru Nagase, Nobuo Yaegashi

    Research output: Contribution to journalArticlepeer-review

    28 Citations (Scopus)

    Abstract

    Objective: Uterine leiomyosarcoma (LMS) has an unfavorable response to standard chemotherapy. A natural occurring compound, curcumin, has been shown to have inhibitory effects on cancers. We previously demonstrated that curcumin reduced uterine LMS cell proliferation by targeting the AKT-mTOR pathway and activating apoptosis. To further explore the anticancer effect of curcumin,we investigated the efficacy of curcumin on autophagy in LMS cells. Methods: Cell proliferation in human uterine LMS cell lines, SKN and SK-UT-1, was assessed after exposure to rapamycin or curcumin. Autophagy was detected byWestern blotting for light chain 3 and sequestosome 1 (SQSTM1/p62) expression. Apoptosis was confirmed by Western blotting for cleaved poly (ADP-ribose) polymerase (PARP). Results: Both rapamycin and curcumin potently inhibited SKN and SK-UT-1 cell proliferation in a dose-dependent manner. Curcumin induced autophagy and apoptosis in SKN and SK-UT-1 cells, whereas rapamycin, a specific mTOR inhibitor, did not. Curcumin increased extracellular signal-regulated kinase 1/2 activity in both SKN and SK-UT-1 cells, whereas PD98059, an MEK1 inhibitor, inhibited both the extracellular signal-regulated kinase 1/2 pathway and curcumin-induced autophagy. Conclusions: These experimental findings suggest that curcumin is a potent inhibitor of cell proliferation in uterine LMS and provide new insights about ongoing signaling events leading to the possible development of a new therapeutic agent.

    Original languageEnglish
    Pages (from-to)803-808
    Number of pages6
    JournalInternational Journal of Gynecological Cancer
    Volume23
    Issue number5
    DOIs
    Publication statusPublished - 2013 Jun

    Keywords

    • Apoptosis
    • Autophagy
    • Curcumin
    • ERK1/2
    • Uterine LMS

    ASJC Scopus subject areas

    • Oncology
    • Obstetrics and Gynaecology

    Fingerprint

    Dive into the research topics of 'Curcumin induces cross-regulation between autophagy and apoptosis in uterine leiomyosarcoma cells'. Together they form a unique fingerprint.

    Cite this