Cultivation of methanogens under low-hydrogen conditions by using the coculture method

Sanae Sakai, Hiroyuki Imachi, Yuji Sekiguchi, I. Cheng Tseng, Akiyoshi Ohashi, Hideki Harada, Yoichi Kamagata

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

We previously reported the isolation of novel methanogens by using a new cultivation method, referred to as the coculture method. Here, we extended our coculture method to various anaerobic environmental samples. As a result, we successfully cultivated some uncharacterized methanogens in coculture enrichments and eventually isolated a new methanogen, within the order Methanomicrobiales.

Original languageEnglish
Pages (from-to)4892-4896
Number of pages5
JournalApplied and environmental microbiology
Volume75
Issue number14
DOIs
Publication statusPublished - 2009 Jul 1

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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    Sakai, S., Imachi, H., Sekiguchi, Y., Tseng, I. C., Ohashi, A., Harada, H., & Kamagata, Y. (2009). Cultivation of methanogens under low-hydrogen conditions by using the coculture method. Applied and environmental microbiology, 75(14), 4892-4896. https://doi.org/10.1128/AEM.02835-08