CUB-domain containing protein 1 represses the epithelial phenotype of pancreatic cancer cells

Shin Miura, Shin Hamada, Atsushi Masamune, Kennichi Satoh, Tooru Shimosegawa

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

The prognosis of pancreatic cancer is dismal due to the frequent metastasis and invasion to surrounding organs. Numerous molecules are involved in the malignant behavior of pancreatic cancer cells, but the entire process remains unclear. Several reports have suggested that CUB-domain containing protein-1 (CDCP1) is highly expressed in pancreatic cancer, but its impact on the invasive growth and the upstream regulator remain elusive. To clarify the role of CDCP1 in pancreatic cancer, we here examined the effects of CDCP1 knockdown on the cell behaviors of pancreatic cancer cells. Knockdown of CDCP1 expression in Panc-1 resulted in reduced cellular migration accompanied by the increased expression of E-cadherin and decreased expression of N-cadherin. Knockdown of CDCP1 attenuated the spheroid formation and resistance against gemcitabine, which are some of the cancer stem cell-related phenotypes. Bone morphogenetic protein 4 (BMP4) was found to induce CDCP1 expression via the extracellular signal regulated kinase pathway, suggesting that CDCP1 has a substantial role in the BMP4-induced epithelial-mesenchymal transition. These results indicate that CDCP1 represses the epithelial phenotype of pancreatic cancer cells.

Original languageEnglish
Pages (from-to)209-218
Number of pages10
JournalExperimental Cell Research
Volume321
Issue number2
DOIs
Publication statusPublished - 2014 Feb 15

Keywords

  • Bone morphogenetic protein 4
  • CUB-domain containing protein 1
  • Epithelial-mesenchymal transition
  • Extracellular signal-regulated kinase
  • Transforming growth factor-β

ASJC Scopus subject areas

  • Cell Biology

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