TY - JOUR
T1 - C3Mab-2
T2 - An Anti-Mouse CCR3 Monoclonal Antibody for Immunocytochemistry
AU - Saito, Masaki
AU - Harigae, Yasuhiro
AU - Li, Guanjie
AU - Asano, Teizo
AU - Tanaka, Tomohiro
AU - Suzuki, Hiroyuki
AU - Kaneko, Mika K.
AU - Kato, Yukinari
N1 - Funding Information:
This research was supported in part by Japan Agency for Medical Research and Development (AMED) under Grant Nos. JP21am0401013 (to Y.K.) and JP21am0101078 (to Y.K.), and by the Japan Society for the Promotion of Science ( JSPS) Grants-in-Aid for Scientific Research (KAKENHI) Grant Nos. 21K06059 (to M.S.), 21K15523 (to T.A.), 21K20789 (to T.T.), 21K07168 (to M.K.K.), and 19K07705 (to Y.K.).
Publisher Copyright:
© Mary Ann Liebert, Inc.
PY - 2022/2/1
Y1 - 2022/2/1
N2 - The C–C motif chemokine receptor 3 (CCR3) is a G protein-coupled receptor activated by eotaxin-1-3, MCP-2-4, and RANTES. CCR3 is associated with allergic diseases and cancer development and is highly expressed in eosinophils, basophils, and cancer cells. Besides, research on the physiological roles of CCR3 is ongoing. Thus, specific monoclonal antibodies (mAbs) for CCR3 would be useful for diagnostic and therapeutic purposes and for unraveling the function of CCR3. We previously developed an anti-mouse CCR3 (mCCR3) mAb (C3Mab-2; rat IgG2b, kappa) using the Cell-Based Immunization and Screening method and showed that C3Mab-2 could detect endogenous and exogenous mCCR3 in flow cytometry. In this study, we showed that C3Mab-2 and its recombinant antibody (recC3Mab-2f) specifically recognized endogenous mCCR3 in P388 (a mouse lymphocyte-like cell line) and J774-1 (a mouse macrophage-like cell line) cells and are usable in immunocytochemistry.
AB - The C–C motif chemokine receptor 3 (CCR3) is a G protein-coupled receptor activated by eotaxin-1-3, MCP-2-4, and RANTES. CCR3 is associated with allergic diseases and cancer development and is highly expressed in eosinophils, basophils, and cancer cells. Besides, research on the physiological roles of CCR3 is ongoing. Thus, specific monoclonal antibodies (mAbs) for CCR3 would be useful for diagnostic and therapeutic purposes and for unraveling the function of CCR3. We previously developed an anti-mouse CCR3 (mCCR3) mAb (C3Mab-2; rat IgG2b, kappa) using the Cell-Based Immunization and Screening method and showed that C3Mab-2 could detect endogenous and exogenous mCCR3 in flow cytometry. In this study, we showed that C3Mab-2 and its recombinant antibody (recC3Mab-2f) specifically recognized endogenous mCCR3 in P388 (a mouse lymphocyte-like cell line) and J774-1 (a mouse macrophage-like cell line) cells and are usable in immunocytochemistry.
KW - CMab-2
KW - Immunocytochemical analysis
KW - Monoclonal antibody
KW - Mouse CCR3
UR - http://www.scopus.com/inward/record.url?scp=85125428647&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85125428647&partnerID=8YFLogxK
U2 - 10.1089/mab.2021.0050
DO - 10.1089/mab.2021.0050
M3 - Article
C2 - 35225661
AN - SCOPUS:85125428647
SN - 2167-9436
VL - 41
SP - 45
EP - 49
JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
IS - 1
ER -
