Crystallization Technique for Strain-free Protein Crystals Using Cross-linked Seed Crystals

Haruhiko Koizumi; Satoshi Uda; Masaru Tachibana; Katsuo Tsukamoto; Kenichi Kojima; Jun Nozawa

doi:10.1021/acs.cgd.6b01136

Crystallization Technique for Strain-free Protein Crystals Using Cross-linked Seed Crystals

Haruhiko Koizumi, Satoshi Uda, Masaru Tachibana, Katsuo Tsukamoto, Kenichi Kojima, Jun Nozawa

Materials Development Division

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

In this study, we show using X-ray diffraction (XRD) rocking-curve measurements that local strain in tetragonal hen egg white (HEW) lysozyme crystals can be removed to a large degree by utilizing seed crystals cross-linked with glutaraldehyde. We also find that misorientation between subgrains and subgrain size decrease when employing cross-linked seed crystals, which suggests that subgrain formation in tetragonal HEW lysozyme crystals can be controlled using this technique. The ideal mosaic structure of proteins that enables the collection of accurate integrated intensities of the diffracted waves is also discussed using tetragonal HEW lysozyme crystals as a model protein.

Original language	English
Pages (from-to)	6089-6094
Number of pages	6
Journal	Crystal Growth and Design
Volume	16
Issue number	10
DOIs	https://doi.org/10.1021/acs.cgd.6b01136
Publication status	Published - 2016 Oct 5

ASJC Scopus subject areas

Chemistry(all)
Materials Science(all)
Condensed Matter Physics

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T1 - Crystallization Technique for Strain-free Protein Crystals Using Cross-linked Seed Crystals

AU - Koizumi, Haruhiko

AU - Uda, Satoshi

AU - Tachibana, Masaru

AU - Tsukamoto, Katsuo

AU - Kojima, Kenichi

AU - Nozawa, Jun

PY - 2016/10/5

Y1 - 2016/10/5

N2 - In this study, we show using X-ray diffraction (XRD) rocking-curve measurements that local strain in tetragonal hen egg white (HEW) lysozyme crystals can be removed to a large degree by utilizing seed crystals cross-linked with glutaraldehyde. We also find that misorientation between subgrains and subgrain size decrease when employing cross-linked seed crystals, which suggests that subgrain formation in tetragonal HEW lysozyme crystals can be controlled using this technique. The ideal mosaic structure of proteins that enables the collection of accurate integrated intensities of the diffracted waves is also discussed using tetragonal HEW lysozyme crystals as a model protein.

AB - In this study, we show using X-ray diffraction (XRD) rocking-curve measurements that local strain in tetragonal hen egg white (HEW) lysozyme crystals can be removed to a large degree by utilizing seed crystals cross-linked with glutaraldehyde. We also find that misorientation between subgrains and subgrain size decrease when employing cross-linked seed crystals, which suggests that subgrain formation in tetragonal HEW lysozyme crystals can be controlled using this technique. The ideal mosaic structure of proteins that enables the collection of accurate integrated intensities of the diffracted waves is also discussed using tetragonal HEW lysozyme crystals as a model protein.

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Crystallization Technique for Strain-free Protein Crystals Using Cross-linked Seed Crystals

Abstract

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