Crystallization of proteins by emulsification-induced concentration in microdroplets

Mao Fukuyama, Aoi Akiyama, Makoto Harada, Tetsuo Okada, Akihide Hibara

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Microdroplet-based protein crystallization using nanodroplet formation induced by spontaneous emulsification is proposed and demonstrated. While the control of the condensation rate of the protein solution required highly accurate flow control or complicated microfabrication in the previous microfluidic protein crystallization systems, this method can achieve it in a simple way; just by changing the surfactant concentration of organic phase in contact with microdroplets. By utilizing this method, the protein crystallization is demonstrated.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1909-1911
Number of pages3
ISBN (Electronic)9780979806483
Publication statusPublished - 2015 Jan 1
Externally publishedYes
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: 2015 Oct 252015 Oct 29

Publication series

NameMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period15/10/2515/10/29

Keywords

  • Microdroplet
  • Protein crystallization
  • Spontaneous emulsification

ASJC Scopus subject areas

  • Control and Systems Engineering

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