TY - JOUR
T1 - Crystallization and X-ray analysis of 2-deoxy-scyllo-inosose synthase, the key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics
AU - Nango, Eriko
AU - Kumasaka, Takashi
AU - Sato, Takao
AU - Tanaka, Nobuo
AU - Kakinuma, Katsumi
AU - Eguchi, Tadashi
PY - 2005/7
Y1 - 2005/7
N2 - A recombinant 2-deoxy-scyllo-inosose synthase from Bacillus circulans has been crystallized at 277 K using PEG 4000 as precipitant. The diffraction pattern of the crystal extends to 2.30 Å resolution at 100 K using synchrotron radiation at the Photon Factory. The crystals are monoclinic and belong to space group P21, with unit-cell parameters a = 80.5, b = 70.4, c = 83.0 Å, β = 117.8°. The presence of two molecules per asymmetric unit gives a crystal volume per protein weight (VM) of 2.89 Å3 Da-1 and a solvent constant of 57.4% by volume.
AB - A recombinant 2-deoxy-scyllo-inosose synthase from Bacillus circulans has been crystallized at 277 K using PEG 4000 as precipitant. The diffraction pattern of the crystal extends to 2.30 Å resolution at 100 K using synchrotron radiation at the Photon Factory. The crystals are monoclinic and belong to space group P21, with unit-cell parameters a = 80.5, b = 70.4, c = 83.0 Å, β = 117.8°. The presence of two molecules per asymmetric unit gives a crystal volume per protein weight (VM) of 2.89 Å3 Da-1 and a solvent constant of 57.4% by volume.
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U2 - 10.1107/S1744309105018841
DO - 10.1107/S1744309105018841
M3 - Article
C2 - 16511136
AN - SCOPUS:33744465535
SN - 1744-3091
VL - 61
SP - 709
EP - 711
JO - Acta Crystallographica Section F:Structural Biology Communications
JF - Acta Crystallographica Section F:Structural Biology Communications
IS - 7
ER -