Crystallization and X-ray analysis of 2-deoxy-scyllo-inosose synthase, the key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics

Eriko Nango, Takashi Kumasaka, Takao Sato, Nobuo Tanaka, Katsumi Kakinuma, Tadashi Eguchi

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

A recombinant 2-deoxy-scyllo-inosose synthase from Bacillus circulans has been crystallized at 277 K using PEG 4000 as precipitant. The diffraction pattern of the crystal extends to 2.30 Å resolution at 100 K using synchrotron radiation at the Photon Factory. The crystals are monoclinic and belong to space group P21, with unit-cell parameters a = 80.5, b = 70.4, c = 83.0 Å, β = 117.8°. The presence of two molecules per asymmetric unit gives a crystal volume per protein weight (VM) of 2.89 Å3 Da-1 and a solvent constant of 57.4% by volume.

Original languageEnglish
Pages (from-to)709-711
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume61
Issue number7
DOIs
Publication statusPublished - 2005 Jul
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

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