Crystallization and preliminary X-ray studies of azoreductases from Bacillus sp. B29

Daiki Ogata, Toshihiko Ooi, Takaaki Fujiwara, Seiichi Taguchi, Isao Tanaka, Min Yao

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Azoreductases from Bacillus sp. B29 are NADH-dependent flavoenzymes which contain a flavin mononucleotide (FMN) as a prosthetic group and exist as homodimers composed of 23 kDa subunits. These enzymes catalyze the reductive degradation of various azo compounds by a ping-pong mechanism. In order to determine the structure-function relationship of the azo-dye reduction mechanism, an X-ray crystallographic study of azoreductases was performed. Selenomethionine-labelled AzrA (SeMet-AzrA) and AzrC were crystallized by the hanging-drop vapour-diffusion method. A crystal of SeMet-AzrA diffracted to 2.0 Å resolution and was determined to belong to space group P212121, with unit-cell parameters a = 56.9, b = 69.0, c = 105.4 Å. The native crystals of AzrC belonged to space group C2, with unit-cell parameters a = 192.0, b = 56.6, c = 105.5 Å, β = 115.7°, and diffracted to 2.21 Å resolution.

Original languageEnglish
Pages (from-to)503-505
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume66
Issue number5
DOIs
Publication statusPublished - 2010 Apr 29
Externally publishedYes

Keywords

  • Azoreductases
  • Bacillus sp. B29
  • Flavoproteins
  • NADH-dependent oxidoreductases

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

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