Crystallization and preliminary X-ray crystallographic studies of the oxidative-stress sensor SoxR and its complex with DNA

Satoshi Watanabe, Akiko Kita, Kazuo Kobayashi, Yasuhiro Takahashi, Kunio Miki

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

SoxR, a member of the MerR family of transcriptional activators, functions as a sensor of oxidative stress. The redox states of the 2Fe-2S cluster of SoxR regulate the activity of SoxR. Here, the crystallization and preliminary crystallographic analysis of SoxR and its complex with DNA are reported. Crystals of SoxR were obtained using PEG 10 000 and glycerol as precipitants. The crystals of SoxR belong to space group P62 or P64, with unit-cell parameters a = b = 80.0, c = 88.1 Å. Crystals of the SoxR-DNA complex were obtained using a 20 bp DNA fragment from a condition containing PEG 10 000 and sodium/potassium tartrate. The crystals of the SoxR-DNA complex belong to space group P6122 or P6522, with unit-cell parameters a = b = 53.5, c = 355.6 Å. Diffraction data were collected to a maximum resolution of 3.2 and 2.7 Å for SoxR and the SoxR-DNA complex, respectively.

Original languageEnglish
Pages (from-to)1275-1277
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume62
Issue number12
DOIs
Publication statusPublished - 2006 Dec 1
Externally publishedYes

Keywords

  • Oxidative stress
  • Sensor proteins
  • Transcriptional activators

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

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