Crystallization and preliminary X-ray crystallographic analysis of a bacterial Asn-transamidosome

Tateki Suzuki, Keitaro Yamashita, Yoshikazu Tanaka, Isao Tanaka, Min Yao

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Most canonical aminoacyl-tRNAs are synthesized directly by their cognate aminoacyl-tRNA synthetases (aaRSs), but glutaminyl-tRNAGln and asparaginyl-tRNAAsn are synthesized indirectly by two-step processes. These processes are catalyzed by the transamidosome, a large ribonucleoprotein particle composed of GatA, GatB, GatC, aaRS and tRNA. In this study, the Asn-transamidosome from Pseudomonas aeruginosa was reconstructed and crystallized by mixing purified GatCAB complex, AspRS and tRNAAsn. The crystal of the Asn-transamidosome belonged to space group P21, with unit-cell parameters a = 93.3, b = 186.0, c = 287.8 Å, β = 93.3°, and diffracted to 3.73 Å resolution. Preliminary X-ray crystallographic analysis showed that the asymmetric unit contained two Asn-transamidosomes, each composed of two GatCABs, one AspRS dimer and two tRNAAsns, indicating that the construction of the current Asn-transamidosome differs from that of Thermus thermophilus.

Original languageEnglish
Pages (from-to)790-793
Number of pages4
JournalActa Crystallographica Section F:Structural Biology Communications
Volume70
Issue number6
DOIs
Publication statusPublished - 2014 Jun
Externally publishedYes

Keywords

  • AspRS
  • GatCAB
  • Pseudomonas aeruginosa
  • aminoacyl-tRNA
  • transamidosome

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

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