Crystallization and preliminary X-ray crystallographic analysis of UDP-glucuronic acid:flavonol-3-O-glucuronosyltransferase (VvGT5) from the grapevine Vitis vinifera

Eiichi Mizohata; Takuma Okuda; Seika Hatanaka; Taisuke Nakayama; Manabu Horikawa; Toru Nakayama; Eiichiro Ono; Tsuyoshi Inoue

doi:10.1107/S1744309112045095

Crystallization and preliminary X-ray crystallographic analysis of UDP-glucuronic acid:flavonol-3-O-glucuronosyltransferase (VvGT5) from the grapevine Vitis vinifera

Eiichi Mizohata, Takuma Okuda, Seika Hatanaka, Taisuke Nakayama, Manabu Horikawa, Toru Nakayama, Eiichiro Ono, Tsuyoshi Inoue

ENG - Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Grapevine (Vitis vinifera) glycosyltransferase 5 (VvGT5) is a UDP-glucuronic acid:flavonol-3-O-glucuronosyltransferase that catalyses the 3-O-specific glucuronosylation of flavonols using UDP-glucuronic acid as a sugar donor to produce flavonol 3-O-glucosides, which are important bioactive phytochemicals. Recombinant VvGT5 expressed in Escherichia coli cells was purified and crystallized by the sitting-drop vapour-diffusion method. A full set of X-ray diffraction data was collected to 2.2 Å Bragg spacing from a single crystal using a synchrotron-radiation source. The crystal was hexagonal, belonging to space group P6122, with unit-cell parameters a = b = 102.70, c = 535.92 Å. The initial phases were determined by the molecular-replacement method.

Original language	English
Pages (from-to)	65-68
Number of pages	4
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	69
Issue number	1
DOIs	https://doi.org/10.1107/S1744309112045095
Publication status	Published - 2013 Jan

Keywords

UGT
VvGT5
flavonoid
flavonol
glycosyltransferase

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics

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Mizohata, E., Okuda, T., Hatanaka, S., Nakayama, T., Horikawa, M., Nakayama, T., Ono, E., & Inoue, T. (2013). Crystallization and preliminary X-ray crystallographic analysis of UDP-glucuronic acid:flavonol-3-O-glucuronosyltransferase (VvGT5) from the grapevine Vitis vinifera. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 69(1), 65-68. https://doi.org/10.1107/S1744309112045095

Crystallization and preliminary X-ray crystallographic analysis of UDP-glucuronic acid:flavonol-3-O-glucuronosyltransferase (VvGT5) from the grapevine Vitis vinifera. / Mizohata, Eiichi; Okuda, Takuma; Hatanaka, Seika; Nakayama, Taisuke; Horikawa, Manabu; Nakayama, Toru; Ono, Eiichiro; Inoue, Tsuyoshi.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 69, No. 1, 01.2013, p. 65-68.

Research output: Contribution to journal › Article › peer-review

Mizohata, E, Okuda, T, Hatanaka, S, Nakayama, T, Horikawa, M, Nakayama, T, Ono, E & Inoue, T 2013, 'Crystallization and preliminary X-ray crystallographic analysis of UDP-glucuronic acid:flavonol-3-O-glucuronosyltransferase (VvGT5) from the grapevine Vitis vinifera', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, vol. 69, no. 1, pp. 65-68. https://doi.org/10.1107/S1744309112045095

Mizohata E, Okuda T, Hatanaka S, Nakayama T, Horikawa M, Nakayama T et al. Crystallization and preliminary X-ray crystallographic analysis of UDP-glucuronic acid:flavonol-3-O-glucuronosyltransferase (VvGT5) from the grapevine Vitis vinifera. Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2013 Jan;69(1):65-68. https://doi.org/10.1107/S1744309112045095

Mizohata, Eiichi ; Okuda, Takuma ; Hatanaka, Seika ; Nakayama, Taisuke ; Horikawa, Manabu ; Nakayama, Toru ; Ono, Eiichiro ; Inoue, Tsuyoshi. / Crystallization and preliminary X-ray crystallographic analysis of UDP-glucuronic acid:flavonol-3-O-glucuronosyltransferase (VvGT5) from the grapevine Vitis vinifera. In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2013 ; Vol. 69, No. 1. pp. 65-68.

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Crystallization and preliminary X-ray crystallographic analysis of UDP-glucuronic acid:flavonol-3-O-glucuronosyltransferase (VvGT5) from the grapevine Vitis vinifera

Abstract

Keywords

ASJC Scopus subject areas

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