Crystallization and preliminary X-ray analysis of reducing-end xylose-releasing exo-oligoxylanase from Bacillus halodurans C-125

Yuji Honda, Shinya Fushinobu, Masafumi Hidaka, Takayoshi Wakagi, Hirofumi Shoun, Motomitsu Kitaoka

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The reducing-end xylose-releasing exo-oligoxylanase (Rex) from Bacillus halodurans C-125, a novel family GH8 glycoside hydrolase, was crystallized by the hanging-drop vapour-diffusion method using 13.6 mg ml-1 purified Rex, 5.6%(v/v) polyethylene glycol 4000, 70 mM sodium acetate pH 4.6 and 30%(v/v) glycerol. Suitable crystals grew after incubation for 5 d at 293 K. The crystals belonged to space group P212121, with unit-cell parameters a = 52.69, b = 86.02, c = 87.92 Å. X-ray diffraction data were collected at a resolution of 1.35 Å.

Original languageEnglish
Pages (from-to)291-292
Number of pages2
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume61
Issue number3
DOIs
Publication statusPublished - 2005 Dec 1
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

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