Crystallization and preliminary X-ray analysis of- Hexachlorocyclohexane dehydrochlorinase LinA from Sphingobium japonicum UT26

Masahiko Okai; Keiko Kubota; Masao Fukuda; Yuji Nagata; Koji Nagata; Masaru Tanokura

doi:10.1107/S1744309109026645

Crystallization and preliminary X-ray analysis of- Hexachlorocyclohexane dehydrochlorinase LinA from Sphingobium japonicum UT26

Masahiko Okai, Keiko Kubota, Masao Fukuda, Yuji Nagata, Koji Nagata, Masaru Tanokura

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

LinA from Sphingobium japonicum UT26 catalyzes two steps of dehydrochlorination from-hexachlorocyclohexane (-HCH) to 1,3,4,6-tetrachloro-1, 4-cyclohexadiene (1,4-TCDN) via-pentachlorocyclohexene (-PCCH). LinA was crystallized by the sitting-drop vapour-diffusion method using PEG 3350 as the precipitant. The crystals belonged to space group P41 or P43, with unit-cell parameters a = b = 68.9, c = 101.9 Å, and diffracted X-rays to 2.25 Å resolution. The crystal contained three molecules in the asymmetric unit.

Original language	English
Pages (from-to)	822-824
Number of pages	3
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	65
Issue number	8
DOIs	https://doi.org/10.1107/S1744309109026645
Publication status	Published - 2009
Externally published	Yes

Keywords

-hexachlorocyclohexane dehydrochlorinase
LinA
Sphingobium japonicum UT26

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics

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10.1107/S1744309109026645

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Crystallization and preliminary X-ray analysis of- Hexachlorocyclohexane dehydrochlorinase LinA from Sphingobium japonicum UT26. / Okai, Masahiko; Kubota, Keiko; Fukuda, Masao; Nagata, Yuji; Nagata, Koji; Tanokura, Masaru.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 65, No. 8, 2009, p. 822-824.

Research output: Contribution to journal › Article › peer-review

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abstract = "LinA from Sphingobium japonicum UT26 catalyzes two steps of dehydrochlorination from-hexachlorocyclohexane (-HCH) to 1,3,4,6-tetrachloro-1, 4-cyclohexadiene (1,4-TCDN) via-pentachlorocyclohexene (-PCCH). LinA was crystallized by the sitting-drop vapour-diffusion method using PEG 3350 as the precipitant. The crystals belonged to space group P41 or P43, with unit-cell parameters a = b = 68.9, c = 101.9 {\AA}, and diffracted X-rays to 2.25 {\AA} resolution. The crystal contained three molecules in the asymmetric unit.",

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author = "Masahiko Okai and Keiko Kubota and Masao Fukuda and Yuji Nagata and Koji Nagata and Masaru Tanokura",

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AU - Okai, Masahiko

AU - Kubota, Keiko

AU - Fukuda, Masao

AU - Nagata, Yuji

AU - Nagata, Koji

AU - Tanokura, Masaru

PY - 2009

Y1 - 2009

N2 - LinA from Sphingobium japonicum UT26 catalyzes two steps of dehydrochlorination from-hexachlorocyclohexane (-HCH) to 1,3,4,6-tetrachloro-1, 4-cyclohexadiene (1,4-TCDN) via-pentachlorocyclohexene (-PCCH). LinA was crystallized by the sitting-drop vapour-diffusion method using PEG 3350 as the precipitant. The crystals belonged to space group P41 or P43, with unit-cell parameters a = b = 68.9, c = 101.9 Å, and diffracted X-rays to 2.25 Å resolution. The crystal contained three molecules in the asymmetric unit.

AB - LinA from Sphingobium japonicum UT26 catalyzes two steps of dehydrochlorination from-hexachlorocyclohexane (-HCH) to 1,3,4,6-tetrachloro-1, 4-cyclohexadiene (1,4-TCDN) via-pentachlorocyclohexene (-PCCH). LinA was crystallized by the sitting-drop vapour-diffusion method using PEG 3350 as the precipitant. The crystals belonged to space group P41 or P43, with unit-cell parameters a = b = 68.9, c = 101.9 Å, and diffracted X-rays to 2.25 Å resolution. The crystal contained three molecules in the asymmetric unit.

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KW - LinA

KW - Sphingobium japonicum UT26

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ER -

Crystallization and preliminary X-ray analysis of- Hexachlorocyclohexane dehydrochlorinase LinA from Sphingobium japonicum UT26

Abstract

Keywords

ASJC Scopus subject areas

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