Crystallization and preliminary X-ray analysis of- Hexachlorocyclohexane dehydrochlorinase LinA from Sphingobium japonicum UT26

Masahiko Okai, Keiko Kubota, Masao Fukuda, Yuji Nagata, Koji Nagata, Masaru Tanokura

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

LinA from Sphingobium japonicum UT26 catalyzes two steps of dehydrochlorination from-hexachlorocyclohexane (-HCH) to 1,3,4,6-tetrachloro-1, 4-cyclohexadiene (1,4-TCDN) via-pentachlorocyclohexene (-PCCH). LinA was crystallized by the sitting-drop vapour-diffusion method using PEG 3350 as the precipitant. The crystals belonged to space group P41 or P43, with unit-cell parameters a = b = 68.9, c = 101.9 Å, and diffracted X-rays to 2.25 Å resolution. The crystal contained three molecules in the asymmetric unit.

Original languageEnglish
Pages (from-to)822-824
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume65
Issue number8
DOIs
Publication statusPublished - 2009

Keywords

  • -hexachlorocyclohexane dehydrochlorinase
  • LinA
  • Sphingobium japonicum UT26

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

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