Crystallization and preliminary X-ray analysis of cellobiose phosphorylase from Cellvibrio gilvus

Masafumi Hidaka, Motomitsu Kitaoka, Kiyoshi Hayashi, Takayoshi Wakagi, Hirofumi Shoun, Shinya Fushinobu

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

A recombinant cellobiose phosphorylase from Cellvibrio gilvus has been prepared and crystallized by the sitting-drop vapour-diffusion method using 10 mg·ml-1 purified enzyme, 1.5 M ammonium sulfate, 0.1 M MES buffer pH 7.0 and 5 mM glucose. A suitable crystal was obtained after 10 d incubation at 298 K. The crystal belongs to space group P21, with unit-cell parameters a = 84.77, b = 98.31, c = 104.04 Å, β = 102.73°. X-ray diffraction data to 2.1 Å resolution have been collected at KEK-PF BL-5A.

Original languageEnglish
Pages (from-to)1877-1878
Number of pages2
JournalActa Crystallographica Section D: Biological Crystallography
Volume60
Issue number10
DOIs
Publication statusPublished - 2004 Oct 1
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology

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