A recombinant cellobiose phosphorylase from Cellvibrio gilvus has been prepared and crystallized by the sitting-drop vapour-diffusion method using 10 mg·ml-1 purified enzyme, 1.5 M ammonium sulfate, 0.1 M MES buffer pH 7.0 and 5 mM glucose. A suitable crystal was obtained after 10 d incubation at 298 K. The crystal belongs to space group P21, with unit-cell parameters a = 84.77, b = 98.31, c = 104.04 Å, β = 102.73°. X-ray diffraction data to 2.1 Å resolution have been collected at KEK-PF BL-5A.
|Number of pages||2|
|Journal||Acta Crystallographica Section D: Biological Crystallography|
|Publication status||Published - 2004 Oct 1|
ASJC Scopus subject areas
- Structural Biology