Abstract
α-L-Arabinofuranosidase from the hyperthermophilic bacterium Thermotoga maritima (Tm-AFase) is an extremely thermophilic enzyme belonging to glycoside hydrolase family 51. It can catalyze the transglycosylation of a novel glycosyl donor, 4,6-dimethoxy-1,3,5-triazin-2-yl (DMT)-β-D-xylopyranoside. In this study we determined the crystal structures of Tm-AFase in substrate-free and complex forms with arabinose and xylose at 1.8-2.3Å resolution to determine the architecture of the substrate binding pocket. Subsite α1 of Tm-AFase is similar to that of α-L-arabinofuranosidase from Geobacillus stearothermophilus, but the substrate binding pocket of Tm-AFase is narrower and more hydrophobic. Possible substrate binding modes were investigated by automated docking analysis.
Original language | English |
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Pages (from-to) | 423-428 |
Number of pages | 6 |
Journal | Bioscience, Biotechnology and Biochemistry |
Volume | 76 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2012 |
Externally published | Yes |
Keywords
- Arabinose
- Glycoside hydrolase family 51
- Thermotoga maritima
- Xylose
- α-L-arabinofuranosidase
ASJC Scopus subject areas
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry