Crystal structure analysis, overexpression and refolding behaviour of a DING protein with single mutation

Zuoqi Gai, Akiyoshi Nakamura, Yoshikazu Tanaka, Nagisa Hirano, Isao Tanaka, Min Yao

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

After crystallization of a certain protein-RNA complex, well diffracting crystals were obtained. However, the asymmetric unit of the crystal was too small to locate any components. Mass spectrometry and X-ray crystal structure analysis showed that it was a member of the DING protein family (HPBP). Surprisingly, the structure of HPBP reported previously was also determined accidentally as a contaminant, suggesting that HPBP has a strong tendency to crystallize. Furthermore, DING proteins were reported to relate in disease. These observations suggest that DING has potential for application in a wide range of research fields. To enable further analyses, a system for preparation of HPBP was constructed. As HPBP was expressed in insoluble form in Escherichia coli, it was unfolded chemically and refolded. Finally, a very high yield preparation method was constructed, in which 43 mg of HPBP was obtained from 1 L of culture. Furthermore, to evaluate the validity of refolding, its crystal structure was determined at 1.03 Å resolution. The determined structure was identical to the native structure, in which two disulfide bonds were recovered correctly and a phosphate ion was captured. Based on these results, it was concluded that the refolded HPBP recovers its structure correctly.

Original languageEnglish
Pages (from-to)854-858
Number of pages5
JournalJournal of Synchrotron Radiation
Volume20
Issue number6
DOIs
Publication statusPublished - 2013 Nov 1
Externally publishedYes

Keywords

  • DING
  • HPBP
  • crystal structure
  • overexpression
  • refolding

ASJC Scopus subject areas

  • Radiation
  • Nuclear and High Energy Physics
  • Instrumentation

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