Cryptococcus neoformans suppresses the activation of bone marrow-derived dendritic cells stimulated with its own DNA, but not with DNA from other fungi

Hideki Yamamoto, Yuzuru Abe, Akiko Miyazato, Daiki Tanno, Misuzu Tanaka, Tomomitsu Miyasaka, Keiko Ishii, Kazuyoshi Kawakami

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

DNA from Cryptococcus neoformans activates bone marrow-derived dendritic cells (BM-DCs) in a TLR9-dependent manner. In this study, we examined the effect of the culture supernatants of C. neoformans on the activation of BM-DCs caused by its own DNA. C. neoformans supernatants suppressed IL-12p40, IL-6 production and CD40 expression by BM-DCs stimulated with its own DNA, but not with CpG-ODN and DNA from Candida albicans, Saccharomyces cerevisiae or Escherichia coli. In a confocal microscopic analysis, C. neoformans DNA was colocalized with LAMP-1, a late endosomal marker, and TLR9. The culture supernatants did not show any apparent suppression of these responses. In a luciferase reporter assay, C. neoformans supernatants inhibited NFκB activation caused by its own DNA. These inhibitory activities were attenuated by treatment with heat or trypsin. These results indicate that C. neoformans secrete certain proteinous molecules that suppress the activation of BM-DCs caused by its own DNA.

Original languageEnglish
Pages (from-to)363-372
Number of pages10
JournalFEMS Immunology and Medical Microbiology
Volume63
Issue number3
DOIs
Publication statusPublished - 2011 Dec

Keywords

  • Cryptococcus neoformans
  • DNA
  • Dendritic cells
  • Inhibition
  • TLR9

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Cryptococcus neoformans suppresses the activation of bone marrow-derived dendritic cells stimulated with its own DNA, but not with DNA from other fungi'. Together they form a unique fingerprint.

Cite this