We found 5 distinct species of Diaphanosoma from a variety of lakes in Japan according to morphological examination and genetic analyses with the DNA sequences of mitochondrial 16S ribosomal RNA and cytochrome c oxidase subunit I genes (mtCOI). Previously, D. brachyurum was thought to occur at temperate regions including Japan; however, we did not find D. brachyurum in Japan. Instead, we found D. cf. amurensis, which inhabited mainly natural habitats of high altitude and latitude, and D. cf. dubium, D. cf. orientalis, and D. cf. macrophthalma, which occurred in a variety of habitats from large natural lakes to small artificial ponds at low altitude. We also found another species from only one pond that possessed mtCOI sequences that matched almost completely with those from North American specimens and thus was probably nonindigenous Diaphanosoma. Concordance between the results of morphological identifications and genetic analyses showed that the DNA barcodes established in this study are useful to identify Diaphanosoma species in Japan and adjacent areas.
- DNA barcode
- Mitochondrial 16s ribosomal RNA gene (mt16S)
- Mitochondrial cytochrome c oxydase subunit i (mtCOI) gene
ASJC Scopus subject areas
- Aquatic Science
- Water Science and Technology