Cryogenic Fluorescence Localization Microscopy of Spectrally Selected Individual FRET Pairs in a Water Matrix

Hiroaki Tabe, Kei Sukenobe, Toru Kondo, Atsunori Sakurai, Minako Maruo, Akari Shimauchi, Mitsuharu Hirano, Shin Nosuke Uno, Mako Kamiya, Yasuteru Urano, Michio Matsushita, Satoru Fujiyoshi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We prepared a pair with a visible-absorbing donor dye and a near-infrared fluorescing acceptor dye. The donor and the acceptor were covalently linked close enough for Förster resonance energy transfer to occur. Under cryogenic conditions at 1.7 K, we observed the fluorescence excitation spectra of the individual pairs in a water matrix. We tested one rhodamine, two Bodipy, and one carbopyronine derivatives as the donor. Among these donors, Bodipy derivatives show the narrowest spectral width of the individuals with respect to the ensemble width. Thus, Bodipy dyes were favorable as the donor for the spectral selection of individual pairs. At 1.7 K, from the several Bodipy-acceptor pairs in the diffraction-limited volume, an individual pair was selected by the fluorescence excitation spectrum of the donor. The spectrally selected pair was localized using the near-infrared fluorescence of the acceptor.

Original languageEnglish
Pages (from-to)6906-6911
Number of pages6
JournalJournal of Physical Chemistry B
Volume122
Issue number27
DOIs
Publication statusPublished - 2018 Jul 12
Externally publishedYes

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

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