TY - JOUR
T1 - Cryogenic Fluorescence Localization Microscopy of Spectrally Selected Individual FRET Pairs in a Water Matrix
AU - Tabe, Hiroaki
AU - Sukenobe, Kei
AU - Kondo, Toru
AU - Sakurai, Atsunori
AU - Maruo, Minako
AU - Shimauchi, Akari
AU - Hirano, Mitsuharu
AU - Uno, Shin Nosuke
AU - Kamiya, Mako
AU - Urano, Yasuteru
AU - Matsushita, Michio
AU - Fujiyoshi, Satoru
N1 - Funding Information:
This work was financially supported by Grants-in-Aid for Scientific Research (16H04094 and 15H03765) and JST/ PRESTO (JPMJPR14FC and JPMJPR14F8). We thank Hiromu Kashida for helpful comments of the design of single-stranded DNA of AT647N−Ax750.
Funding Information:
This work was financially supported by Grants-in-Aid for Scientific Research (16H04094 and 15H03765) and JST/ PRESTO (JPMJPR14FC and JPMJPR14F8). We thank Hiromu Kashida for helpful comments of the design of single-stranded DNA of AT647N-Ax750.
Publisher Copyright:
Copyright © 2018 American Chemical Society.
PY - 2018/7/12
Y1 - 2018/7/12
N2 - We prepared a pair with a visible-absorbing donor dye and a near-infrared fluorescing acceptor dye. The donor and the acceptor were covalently linked close enough for Förster resonance energy transfer to occur. Under cryogenic conditions at 1.7 K, we observed the fluorescence excitation spectra of the individual pairs in a water matrix. We tested one rhodamine, two Bodipy, and one carbopyronine derivatives as the donor. Among these donors, Bodipy derivatives show the narrowest spectral width of the individuals with respect to the ensemble width. Thus, Bodipy dyes were favorable as the donor for the spectral selection of individual pairs. At 1.7 K, from the several Bodipy-acceptor pairs in the diffraction-limited volume, an individual pair was selected by the fluorescence excitation spectrum of the donor. The spectrally selected pair was localized using the near-infrared fluorescence of the acceptor.
AB - We prepared a pair with a visible-absorbing donor dye and a near-infrared fluorescing acceptor dye. The donor and the acceptor were covalently linked close enough for Förster resonance energy transfer to occur. Under cryogenic conditions at 1.7 K, we observed the fluorescence excitation spectra of the individual pairs in a water matrix. We tested one rhodamine, two Bodipy, and one carbopyronine derivatives as the donor. Among these donors, Bodipy derivatives show the narrowest spectral width of the individuals with respect to the ensemble width. Thus, Bodipy dyes were favorable as the donor for the spectral selection of individual pairs. At 1.7 K, from the several Bodipy-acceptor pairs in the diffraction-limited volume, an individual pair was selected by the fluorescence excitation spectrum of the donor. The spectrally selected pair was localized using the near-infrared fluorescence of the acceptor.
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U2 - 10.1021/acs.jpcb.8b03977
DO - 10.1021/acs.jpcb.8b03977
M3 - Article
C2 - 29889523
AN - SCOPUS:85048725835
VL - 122
SP - 6906
EP - 6911
JO - Journal of Physical Chemistry B
JF - Journal of Physical Chemistry B
SN - 1520-6106
IS - 27
ER -