Cryo-EM analysis provides new mechanistic insight into ATP binding to Ca2+-ATPase SERCA2b

Yuxia Zhang, Satoshi Watanabe, Akihisa Tsutsumi, Hiroshi Kadokura, Masahide Kikkawa, Kenji Inaba

Research output: Contribution to journalArticlepeer-review

Abstract

Sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) 2b is a ubiquitous SERCA family member that conducts Ca2+ uptake from the cytosol to the ER. Herein, we present a 3.3 Å resolution cryo-electron microscopy (cryo-EM) structure of human SERCA2b in the E1·2Ca2+ state, revealing a new conformation for Ca2+-bound SERCA2b with a much closer arrangement of cytosolic domains than in the previously reported crystal structure of Ca2+-bound SERCA1a. Multiple conformations generated by 3D classification of cryo-EM maps reflect the intrinsically dynamic nature of the cytosolic domains in this state. Notably, ATP binding residues of SERCA2b in the E1·2Ca2+ state are located at similar positions to those in the E1·2Ca2+-ATP state; hence, the cryo-EM structure likely represents a preformed state immediately prior to ATP binding. Consistently, a SERCA2b mutant with an interdomain disulfide bridge that locks the closed cytosolic domain arrangement displayed significant autophosphorylation activity in the presence of Ca2+. We propose a novel mechanism of ATP binding to SERCA2b.

Original languageEnglish
Article numbere108482
JournalEMBO Journal
Volume40
Issue number19
DOIs
Publication statusPublished - 2021 Oct 1

Keywords

  • ATP binding
  • cryo-EM structure
  • SERCA2b
  • single-particle analysis

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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