Crucial role of ATP-bound Sse1 in Upf1-dependent degradation of the truncated product

Takato Sugiyama, Risa Nobuta, Koji Ando, Yasuko Matsuki, Toshifumi Inada

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Up-frameshift (Upf) complex facilitates the degradation of aberrant mRNAs containing a premature termination codon (PTC) and its products in yeast. Here we report that Sse1, a member of the Hsp110 family, and Hsp70 play a crucial role in Upf-dependent degradation of the truncated FLAG-Pgk1-300 protein derived from PGK1 mRNA harboring a PTC at codon position 300. Sse1 was required for Upf-dependent rapid degradation of the FLAG-Pgk1-300. FLAG-Pgk1-300 was significantly destabilized in ATP hydrolysis defective sse1-1 mutant cells than in wild type cells. Consistently, Sse1 and Hsp70 reduced the level of an insoluble form of FLAG-Pgk1-300. We propose that the Sse1/Hsp70 complex maintains the solubility of FLAG-Pgk1-300, thereby stimulating its Upf-dependent degradation by the proteasomes.

Original languageEnglish
Pages (from-to)122-128
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume488
Issue number1
DOIs
Publication statusPublished - 2017 Jun 17

Keywords

  • Hsp70/Sse1 complex
  • Nonsense-mediated decay
  • Proteasome
  • Ubiquitination

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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